Rat glucocorticoid receptor-alpha (GR-α) ELISA kit-Quantitative sandwich

Cat. No.: 0126WXX-292

Assay Type: Quantitative sandwich ELISA

Target Species: Rat

Assay Target: GR-α

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Rat glucocorticoid receptor-alpha (GR-α) ELISA kit-Quantitative sandwich

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Product Overview

Description: Rat glucocorticoid receptor-alpha (GR-α) ELISA kit is an ELISA-based in vitro research tool designed specifically for the quantitative detection of GR-α in rat samples with a range of 0.05-20 ng/mL and a minimum detectable dose (sensitivity) of 0.026 ng/mL. The kit is highly sensitive and easy to use.

Assay Principle: The microplate is pre-coated with a rat GR-α antibody. Rat GR-α present in the sample binds to the immobilized capture antibodies. Next, a biotinylated rat GR-α detection antibody is added. Streptavidin-HRP is then introduced to bind the biotinylated antibody. After incubation, unbound reagents are washed away. A substrate solution is introduced, and color develops in proportion to the amount of rat GR-α present. The reaction is terminated with an acidic stop solution, and the final absorbance is measured at 450 nm.

Background: GR-α is a major nuclear receptor that specifically binds glucocorticoids (such as cortisol) and mediates their diverse biological effects in the body. GR-α functions in two distinct ways: it acts as a transcription factor, directly binding to specific sequences on genes to regulate gene expression; and it serves as a regulator of other transcription factors, influencing processes such as inflammation, cell proliferation, and differentiation. GR-α is the key molecule linking cortisol signaling to the pathophysiology of obesity.

Synonyms: GR-alpha

Applications: Rat glucocorticoid receptor-alpha (GR-α) ELISA kit is used to quantify GR-α in serum, plasma, cell culture supernates, cell lysates, and tissue homogenates of rat, providing data to support research in a wide range of areas, including signal transduction, metabolic pathway, endocrinology, hormone metabolism, obesity, and others.

Research Area: Signal transduction; Metabolic pathway; Endocrinology; Hormone metabolism; Obesity

Specification

Sample Type: Serum; Plasma; Cell culture supernates; Cell lysates; Tissue homogenates

Detection Range: 0.05-20 ng/mL

Sensitivity: 0.026 ng/mL

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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