Rat glucagon (GCG) ELISA kit (1.0 pg/mL)

Cat. No.: 0126WXX-80

Assay Type: Quantitative competitive ELISA

Target Species: Rat

Assay Target: GCG

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Rat glucagon (GCG) ELISA kit (1.0 pg/mL)

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Product Overview

Description: Rat glucagon (GCG) ELISA kit (1.0 pg/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of GCG in rat samples with a minimum detectable dose (sensitivity) of 1.0 pg/mL. The kit is highly sensitive and easy to use.

Assay Principle: This kit employs a competitive enzyme immunoassay technique with a polyclonal anti-GCG antibody pre-coated onto the microplate. GCG from the sample and a fixed amount of GCG-HRP conjugate compete for binding sites on the antibody. After a one-hour incubation, unbound components are washed away. A substrate is added, producing a blue color that turns yellow upon the addition of a stop solution. The intensity is measured at 450 nm. The color intensity is inversely proportional to the GCG concentration in the sample, with higher sample concentrations resulting in a lighter color.

Background: Glucagon is a hormone secreted by A cells of the islets of Langerhans, playing a pivotal role in glucose metabolism and homeostasis. Its primary function is to regulate blood glucose levels by promoting gluconeogenesis and inhibiting glycolysis. The dynamic equilibrium between glucagon and insulin is crucial for maintaining healthy metabolism. The detection of glucagon and analysis of its associated pathways provide key insights into understanding and treating obesity.

Synonyms: GLP1; GLP2; GRPP; Glicentin-related polypeptide; Glucagen; Oxyntomodulin; Incretin hormone; GC

Applications: Rat glucagon (GCG) ELISA kit (1.0 pg/mL) is used to quantify GCG in serum, plasma, cell culture supernatants, body fluid, and tissue homogenate of rat, providing data to support research in a wide range of areas, including endocrinology, obesity, metabolism, and others.

Research Area: Endocrinology; Obesity; Metabolism

Specification

Sample Type: Serum; Plasma; Cell culture supernatants; Body fluid; Tissue homogenate

Detection Range: 100-2500 pg/mL

Sensitivity: 1.0 pg/mL

Precision (Intra-assay): CV<10%

Precision (Inter-assay): CV<12%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Reaction Time: 1.5 h

Recovery: 92-101%

Storage: Store at 2-8°C.

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