Rat estrogen receptor (ER) ELISA kit (0.312-20 ng/mL, 0.06 ng/mL)

Cat. No.: OB0625WXX-722

Assay Type: Quantitative sandwich ELISA

Target Species: Rat

Assay Target: ER

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Rat estrogen receptor (ER) ELISA kit (0.312-20 ng/mL, 0.06 ng/mL)

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Product Overview

Description: Rat estrogen receptor (ER) ELISA kit (0.312-20 ng/mL, 0.06 ng/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of ER in rat with a range of 0.312-20 ng/mL and a specificity of 0.06 ng/mL.

Assay Principle: The pre-coated antibody is a monoclonal antibody to rat ER, and the detection antibody is a biotin-labeled polyclonal antibody. Samples and biotin-labeled antibodies are added to the ELISA plate wells and rinsed with PBS or TBS. Avidin-peroxidase conjugate is then sequentially added to the ELISA wells; the reactants are thoroughly rinsed with PBS or TBS and then colored with TMB substrate. TMB turns blue when catalyzed by peroxidase and finally turns yellow in the presence of acid. The shade of color is positively correlated with the detection factor in the sample.

Background: ER is a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. Estrogens and their receptors play roles in sexual development, reproductive functions, bone, and other tissues. ERs are associated with various pathological processes.

Synonyms: Estradiol receptor; Oestrogen receptor; Receptors, Estrogen

Applications: Rat estrogen receptor (ER) ELISA kit (0.312-20 ng/mL, 0.06 ng/mL) is used to quantify ER in rat serum, plasma, cell culture supernatant, and tissue samples of rat, providing data to support a wide range of studies.

Research Area: Endocrinology

Specification

Sample Type: Rat serum; Plasma; Cell culture supernatant; Organizations

Detection Range: 0.312-20 ng/mL

Sensitivity: 0.06 ng/mL

Precision (Intra-assay): CV≤8%

Precision (Inter-assay): CV≤12%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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