Rat alpha-ketoglutarate-dependent dioxygenase (FTO) ELISA kit (0.1 ng/mL)

Cat. No.: 0126WXX-38

Assay Type: Quantitative competitive ELISA

Target Species: Rat

Assay Target: FTO

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Rat alpha-ketoglutarate-dependent dioxygenase (FTO) ELISA kit (0.1 ng/mL)

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Product Overview

Description: Rat alpha-ketoglutarate-dependent dioxygenase (FTO) ELISA kit (0.1 ng/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of FTO in rat samples with a minimum detectable dose (sensitivity) of 0.1 ng/mL. The kit is highly sensitive and easy to use.

Assay Principle: The FTO ELISA kit applies a competitive enzyme immunoassay format utilizing a monoclonal anti-FTO antibody and an FTO-HRP conjugate. The assay sample and buffer are incubated with the FTO-HRP conjugate in the pre-coated microplate for one hour. Endogenous FTO and the FTO-HRP conjugate compete for the limited binding sites on the immobilized monoclonal antibody. After the incubation period, the plate is decanted and washed five times. An HRP substrate is then added to produce a blue colored product, which shifts to yellow after the reaction is terminated with the stop solution. The color intensity, measured at 450 nm, is inversely proportional to the FTO concentration. By plotting a standard curve of O.D. versus concentration, the FTO concentration in each sample is accurately determined.

Background: FTO is a crucial dioxygenase primarily responsible for repairing alkylated DNA and RNA through oxidative demethylation. It exhibits the highest activity toward single-stranded RNA containing 3-methyluracil and requires molecular oxygen, α-ketoglutarate, and iron as cofactors. Beyond its role in nucleic acid repair, FTO participates in regulating systemic metabolic rate, energy expenditure, and energy homeostasis, thereby influencing body composition and adipose tissue accumulation. Defects in this protein are associated with various disorders, including growth retardation, developmental delays, and premature mortality. The relationship between FTO and obesity represents a highly significant research area, given that FTO is currently one of the strongest known genetic associates of obesity. Research indicates that FTO influences body weight by regulating energy balance and fat accumulation. Its mechanisms involve modulating appetite and satiety signaling pathways, as well as influencing lipolysis and lipogenesis processes. Specific FTO gene variants have been demonstrated to be significantly associated with higher BMI and obesity risk.

Synonyms: GDFD; IFEX9; ALKBH9; BMIQ14; KIAA1752; Fat mass and obesity-associated protein; FTO, alpha-ketoglutarate dependent dioxygenase; EC 1.14.11.-

EC NO.: 1.14.11.-

Applications: Rat alpha-ketoglutarate-dependent dioxygenase (FTO) ELISA kit (0.1 ng/mL) is used to quantify FTO in serum, plasma, cell culture supernatants, body fluid, and tissue homogenate of rat, providing data to support research in a wide range of areas, including enzyme & kinase, obesity, and others.

Research Area: Enzyme & kinase; Obesity

Specification

Sample Type: Serum; Plasma; Cell culture supernatants; Body fluid; Tissue homogenate

Detection Range: 1.0-25 ng/mL

Sensitivity: 0.1 ng/mL

Cross-reactivity: No significant cross-reactivity or interference was observed.

Recovery: 94-103%

Storage: Store at 2-8°C.

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