Rabbit tartrate resistant acid phosphatase 5b (TRACP 5b) ELISA kit (0.312-20 mIU/mL)

Cat. No.: 0126WXX-1995

Assay Type: Quantitative sandwich ELISA

Target Species: Rabbit

Assay Target: TRACP 5b

Size:

Rabbit tartrate resistant acid phosphatase 5b (TRACP 5b) ELISA kit (0.312-20 mIU/mL)

INQUIRY Datasheet MSDS

Add to basket

Product Overview

Description: Rabbit tartrate resistant acid phosphatase 5b (TRACP 5b) ELISA kit (0.312-20 mIU/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of TRACP 5b in rabbit samples with a range of 0.312-20 mIU/mL and a minimum detectable dose (sensitivity) of <0.06 mIU/mL. The kit is highly sensitive and easy to use.

Assay Principle: The microplate is pre-coated with a rabbit TRACP-5b monoclonal antibody, and the detection agent is a biotin-labeled polyclonal antibody. Samples and biotinylated antibodies are added to the ELISA wells and washed with PBS or TBS. Subsequently, avidin-peroxidase conjugates are introduced. After a thorough wash step, TMB substrate is added for coloration. The TMB product turns blue through peroxidase catalysis and shifts to yellow upon acidification. The resulting color intensity is positively correlated with the concentration of the analyte in the specimen.

Background: TRACP 5B, a metallophosphoesterase located at 19p13.2, encodes a tartrate-resistant acid phosphatase (EC 3.1.3.2) essential for bone resorption and the negative regulation of inflammatory cytokines, including TNF and interleukin-1 beta. By dephosphorylating osteopontin and bone sialoprotein, it maintains skeletal integrity and immune homeostasis; consequently, inactivating mutations lead to SPENCDI, while its elevated expression serves as a biomarker for various leukemias and Gaucher disease. In the context of metabolic health, TRACP 5B is increasingly recognized for its role in obesity-driven chronic inflammation, as its regulation of osteopontin signaling and macrophage activity directly influences the adipose tissue remodeling and systemic insulin resistance associated with excessive weight gain.

Synonyms: ACP5; TRAP5b; SPENCDI; TRACP-5b

Applications: Rabbit tartrate resistant acid phosphatase 5b (TRACP 5b) ELISA kit (0.312-20 mIU/mL) is used to quantify TRACP 5b in rabbit serum, plasma, and cell culture supernatant of rabbit, providing data to support research in a wide range of areas, including signal transduction, metabolism, obesity, and others.

Research Area: Signal transduction; Metabolism; Obesity