Rabbit lipopolysaccharide binding protein (LBP) ELISA kit (1.56-100 ng/mL)

Cat. No.: 0126WXX-774

Assay Type: Quantitative sandwich ELISA

Target Species: Rabbit

Assay Target: LBP

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Rabbit lipopolysaccharide binding protein (LBP) ELISA kit (1.56-100 ng/mL)

INQUIRY Datasheet MSDS

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Product Overview

Description: Rabbit lipopolysaccharide binding protein (LBP) ELISA kit (1.56-100 ng/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of LBP in rabbit samples with a range of 1.56-100 ng/mL and a minimum detectable dose (sensitivity) of <0.5 ng/mL. The kit is highly sensitive and easy to use.

Assay Principle: The microplate is pre-coated with a rabbit LBP monoclonal antibody, and a biotin-labeled polyclonal antibody is used for detection. Samples and detection antibodies are added and washed with PBS or TBS. Avidin-peroxidase conjugates are then added. Following a thorough wash, TMB substrate is introduced. The resulting blue product shifts to yellow upon acidification. The color depth and the concentration of the target factors in the sample are positively correlated.

Background: LBP is a secreted protein primarily present in the extracellular space. Its core function is to bind bacterial lipopolysaccharide (LPS), a glycolipid found on the outer membrane of all Gram-negative bacteria. LBP is significant in obesity research because it plays a pivotal role in the link between inflammation, gut microbiota, and metabolic health. By studying LBP's mechanisms of action and its level changes, scientists can better understand how obesity leads to systemic inflammation and insulin resistance, potentially developing therapies targeting LBP or its associated signaling pathways.

Synonyms: LPS-binding protein

Applications: Rabbit lipopolysaccharide binding protein (LBP) ELISA kit (1.56-100 ng/mL) is used to quantify LBP in serum, plasma, and cell culture supernatant of rabbit, providing data to support research in a wide range of areas, including Infection immunity, obesity, etc.

Research Area: Infection immunity; Obesity

Specification

Sample Type: Rabbit serum; Plasma; Cell culture supernatant

Detection Range: 1.56-100 ng/mL

Sensitivity: <0.5 ng/mL

Precision (Intra-assay): CV≤8%

Precision (Inter-assay): CV≤12%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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