Rabbit insulin (INS) ELISA kit (8-140 μIU/mL)

Cat. No.: 0126WXX-615

Assay Type: Quantitative ELISA

Target Species: Rabbit

Assay Target: INS

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Rabbit insulin (INS) ELISA kit (8-140 μIU/mL)

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Product Overview

Description: Rabbit insulin (INS) ELISA kit (8-140 μIU/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of INS in rabbit samples with a range of 8-140 μIU/mL and a minimum detectable dose (sensitivity) of 5 μIU/mL. The kit is highly sensitive and easy to use.

Assay Principle: This INS ELISA Kit utilizes a direct sandwich enzyme immunoassay technique. The provided microtiter plate is pre-coated with an antibody specific to INS. Standards or samples are added to the wells along with a HRP-conjugated detection antibody specific for INS and incubated to form an immune complex. Following incubation, substrate solutions A and B are added to each well; a color change occurs only in wells containing the INS-HRP complex. The reaction is terminated with a sulphuric acid solution, and the absorbance is measured spectrophotometrically at 450 nm ± 2 nm. The concentration of INS in the samples is determined by comparing the optical density (O.D.) to a standard curve.

Background: INS is a hormone composed of an A chain and a B chain linked by two disulfide bonds. Secreted by the pancreas, it functions as a key regulator of metabolism within the body. Its primary role is to lower blood glucose levels by promoting glucose uptake into cells. INS is fundamentally linked to obesity through its role in energy metabolism and its relationship with insulin resistance. By targeting the insulin receptor signaling pathway and the diverse metabolic processes it regulates, researchers can develop novel anti-obesity therapies.

Synonyms: Pancreatic insulin

Applications: Rabbit insulin (INS) ELISA kit (8-140 μIU/mL) is used to quantify INS in serum, plasma, and tissue homogenates of rabbit, providing data to support research in a wide range of areas, including metabolic pathway, endocrinology, hormone metabolism, obesity, etc.

Research Area: Metabolic pathway; Endocrinology; Hormone metabolism; Obesity

Specification

Sample Type: Serum; Plasma; Tissue homogenates

Sample Volume: 50-100 μL

Detection Range: 8-140 μIU/mL

Sensitivity: 5 μIU/mL

Precision (Intra-assay): CV<8%

Precision (Inter-assay): CV<10%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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