Rabbit apolipoprotein C-III (apo-CIII) ELISA kit

Cat. No.: OB0625WXX-164

Assay Type: Quantitative competitive ELISA

Target Species: Rabbit

Assay Target: apo-CIII

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Rabbit apolipoprotein C-III (apo-CIII) ELISA kit

INQUIRY Datasheet MSDS

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Product Overview

Description: Rabbit apolipoprotein C-III (apo-CIII) ELISA kit is an ELISA-based in vitro research tool designed specifically for the quantitative detection of apo-CIII in rabbits with a specificity of 1.0 ng/mL.

Assay Principle: The assay samples and buffer are incubated with Apo-CIII-HRP conjugate in pre-coated plates for one hour. At the end of the incubation period, the wells are poured out and washed five times. The wells are then incubated with the substrate for the HRP enzyme. The product of the reaction of the enzyme with the substrate forms a blue colored complex. Finally, the reaction is stopped by the addition of a stop solution, which turns the solution yellow. The color intensity is measured spectrophotometrically at 450 nm on a microplate reader. The color intensity is inversely proportional to the concentration of Apo-CIII because Apo-CIII and Apo-CIII-HRP conjugates in the sample compete for the binding site of the anti-Apo-CIII antibody. Since there are a limited number of binding sites, as more sites are occupied by Apo-CIII in the sample, fewer sites remain to bind the Apo-CIII-HRP conjugate. A standard curve is plotted to relate the color intensity (O.D.) to the concentration of the standard. The Apo-CIII concentration in each sample is extrapolated from this standard curve.

Background: Apolipoprotein C-III is secreted by the liver and small intestine. It is a very low-density lipoprotein (VLDL) protein that inhibits lipoprotein lipase and hepatic lipase. Studies have found that it plays a key role in the metabolism of triglyceride-rich lipoproteins (TRLs).

Synonyms: ApoC-III ; ApoCIII; Apo C-III; Apo C III; Apolipoprotein CIII; APOC3

Applications: Rabbit apolipoprotein C-III (apo-CIII) ELISA kit is an important tool for the quantitative detection of apo-CIII in various biological samples (serum, plasma, cell culture supernatants, body fluid, tissue homogenate) of rabbits.

Research Area: Lipid metabolism; Fatty acid biosynthesis; Obesity research

Specification

Sample Type: Serum; Plasma; Cell culture supernatants; Body fluid; Tissue homogenate

Sensitivity: 1.0 ng/mL

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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