Porcine peroxisome proliferator-activated receptor α (PPARα) ELISA kit (0.156-10 ng/mL)

Cat. No.: 0126WXX-1496

Assay Type: Quantitative sandwich ELISA

Target Species: Porcine

Assay Target: PPARα

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Porcine peroxisome proliferator-activated receptor α (PPARα) ELISA kit (0.156-10 ng/mL)

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Product Overview

Description: Porcine peroxisome proliferator-activated receptor α (PPARα) ELISA kit (0.156-10 ng/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of PPARα in porcine samples with a range of 0.156-10 ng/mL and a minimum detectable dose (sensitivity) of 0.05 ng/mL. The kit is highly sensitive and easy to use.

Assay Principle: This assay utilizes a double-antibody sandwich ELISA technique. The microplate is pre-coated with a human PPARα monoclonal antibody, while a biotin-labeled polyclonal antibody serves as the detection agent. Samples and biotinylated detection antibodies are added to the ELISA plate wells and subsequently washed with PBS or TBS. Avidin-peroxidase conjugates are then introduced to the wells. After the reactants are thoroughly washed with PBS or TBS, a TMB substrate is used for color development. The TMB turns blue through peroxidase catalysis and finally shifts to yellow under the action of an acidic stop solution. The color depth is positively correlated with the concentration of the target factors in the samples.

Background: PPAR-alpha, a member of the NR1 nuclear hormone receptor subfamily located at chromosomal position 22q13.31, serves as a master ligand-activated transcription factor and a cornerstone regulator of lipid homeostasis in obesity research. Primarily localized within the nucleus, its transactivation activity—requiring heterodimerization with RXRA and being antagonized by NR2C2—is triggered by various endogenous ligands such as 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphocholine (16:0/18:1-GPC), satiety-regulating lipids like oleylethanolamide, and exogenous peroxisome proliferators. Beyond its fundamental role in activating the peroxisomal beta-oxidation pathway through genes such as ACOX1 and P450, PPAR-alpha orchestrates a complex physiological network including fatty acid transport, lipoprotein metabolism, and the negative regulation of appetite and glycolysis. Its multifaceted molecular functions—ranging from zinc ion binding to steroid hormone-mediated signaling—integrate critical biological processes such as insulin stimulus response, gluconeogenesis, and circadian rhythm regulation, making it an essential target for understanding and intervening in metabolic disorders and chronic obesity.

Synonyms: Peroxisome proliferator activated receptor alpha; NR1C1; PPAR-A; HPPAR; Nuclear receptor subfamily 1 group C member 1; PPARa; PPARA; PPAR-alpha

Applications: Porcine peroxisome proliferator-activated receptor α (PPARα) ELISA kit (0.156-10 ng/mL) is used to quantify PPARα in serum, plasma, cell culture supernatant, and tissues of porcine, providing data to support research in a wide range of areas, including signal transduction, developmental science, obesity, and others.

Research Area: Signal transduction; Developmental science; Obesity