Porcine nicotinamide phosphoribosyltransferase (NAMPT) ELISA kit (3.12-200 ng/mL)

Cat. No.: 0126WXX-1056

Assay Type: Quantitative ELISA

Target Species: Porcine

Assay Target: NAMPT

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Porcine nicotinamide phosphoribosyltransferase (NAMPT) ELISA kit (3.12-200 ng/mL)

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Product Overview

Description: Porcine nicotinamide phosphoribosyltransferase (NAMPT) ELISA kit (3.12-200 ng/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of NAMPT in porcine samples with a range of 3.12-200 ng/mL and a minimum detectable dose (sensitivity) of <1.2 ng/mL. The kit is highly sensitive and easy to use.

Assay Principle: The microtiter plate provided in this kit has been pre-immobilized with an antibody specific to the target antigen. Standards or samples are introduced into the designated microtiter plate wells along with a biotin-conjugated detection antibody preparation specific for the target antigen. Subsequently, avidin-conjugated HRP is added to each microplate well and incubated. After the addition of TMB substrate solution, color development occurs exclusively in those wells containing the target antigen-biotin-avidin sandwich complex. A sulphuric acid solution terminates the enzymatic reaction, and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of the target antigen in the samples is then determined by comparing the O.D. of the samples to the established standard curve.

Background: NAMPT is the rate-limiting enzyme for nicotinamide adenine dinucleotide (NAD⁺) synthesis, catalyzing the conversion of nicotinamide to nicotinamide mononucleotide (NMN), which is then used to synthesize NAD⁺. NAMPT exists in two forms: the intracellular isoform (iNAMPT), which maintains intracellular NAD⁺ pools, and the secreted isoform (eNAMPT or Visfatin). The relevance of NAMPT to obesity research lies in NAD⁺ serving as a key cofactor for metabolic signaling and enzyme activity (e.g., Sirtuins). NAMPT activity serves as a central node linking nutritional status, energy metabolism, and aging. The secreted form of eNAMPT functions as an adipokine; studies have identified its abnormal expression in adipose tissue and serum of obese patients, suggesting potential roles in regulating insulin sensitivity and chronic inflammation.

Synonyms: Pbef; Pbef1; AI314458; AI480535; Visfatin; NAmPRTase; 1110035O14Rik; Pre-B-cell colony-enhancing factor 1 homolog; PBEF; Visfatin; EC 2.4.2.12

EC NO.: 2.4.2.12

Applications: Pig nicotinamide phosphoribosyltransferase (NAMPT) ELISA kit (3.12-200 ng/mL) is used to quantify NAMPT in serum, plasma, whole blood, tissue, saliva, urine, feces, and other fluids of pig, providing data to support research in a wide range of areas, including mitochondrial function, inflammation, obesity, etc.

Research Area: Mitochondrial function; Inflammation; Obesity

Specification

Sample Type: Serum; Plasma; Whole blood; Other fluid; Tissue; Saliva; Urine; Feces

Detection Range: 3.12-200 ng/mL

Sensitivity: <1.2 ng/mL

Precision (Intra-assay): CV≤5.6%

Precision (Inter-assay): CV≤9.8%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at -20°C.

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