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Porcine leptin receptor (LEPR) ELISA kit (5.0-100 ng/mL)
Cat. No.:
0126WXX-975
Assay Type:
Quantitative ELISA
Target Species:
Porcine
Assay Target:
LEPR
Size:
Add to basket
Product Overview
Description:
Porcine leptin receptor (LEPR) ELISA kit (5.0-100 ng/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of LEPR in porcine samples with a range of 5.0-100 ng/mL and a minimum detectable dose (sensitivity) of 1.0 ng/mL. The kit is highly sensitive and easy to use.
Assay Principle:
The LEPR ELISA kit utilizes a quantitative sandwich enzyme immunoassay technique. The microtiter plate is pre-coated with a monoclonal antibody specific for LEPR. Samples or standards are added, and any LEPR present is captured. An HRP-conjugated polyclonal antibody specific for LEPR is then introduced to form the sandwich complex. After incubation and a wash to remove unbound components, substrate solutions are added. The resulting color change is stopped with sulphuric acid and measured at 450 nm. The LEPR concentration is then interpolated from the standard curve.
Background:
LEPR, as a peptide hormone receptor, is responsible for receiving leptin signals. When leptin binds to LEPR, it initiates a series of intracellular cascades, including the positive regulation of the JAK-STAT signaling pathway and the positive regulation of the PI3K signaling pathway. These pathways are crucial for translating leptin signals into biological responses, such as appetite suppression and metabolic regulation. The relationship between LEPR and obesity is paramount. When leptin functions as the “satiety hormone,” LEPR acts as the “satiety switch” in the brain and other tissues. Without functional receptors, leptin signals cannot be received regardless of how high leptin concentrations become.
Synonyms:
CD295; LEPR; OBR; LR
Applications:
Porcine leptin receptor (LEPR) ELISA kit (5.0-100 ng/mL) is used to quantify LEPR in serum, plasma, cell culture supernatants, body fluid, tissue homogenate of porcine, providing data to support research in a wide range of areas, including signal transduction, CD & adhesion molecule, metabolic pathway, endocrinology, obesity, etc.
Research Area:
Signal transduction; CD & Adhesion molecule; Metabolic pathway; Endocrinology; Obesity
Specification
Sample Type:
Serum; Plasma; Cell culture supernatants; Body fluid; Tissue homogenate
Detection Range:
5.0-100 ng/mL
Sensitivity:
1.0 ng/mL
Cross-reactivity:
No significant cross-reactivity or interference was observed.
Storage:
Store at 2-8°C.