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Pig colipase (CLPS) ELISA kit (0.156-10 ng/mL)
Cat. No.:
OB0625WXX-318
Assay Type:
Quantitative sandwich ELISA
Target Species:
Pig
Assay Target:
CLPS
Size:
Add to basket
Product Overview
Description:
Pig colipase (CLPS) ELISA kit (0.156-10 ng/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of CLPS in pig with a range of 0.156-10 ng/mL and a specificity of 0.1 ng/mL.
Assay Principle:
An antibody specific for CLPS has been pre-coated on the microtiter plate. Standards and samples are pipetted into the wells, and any CLPS present is bound by the immobilized antibody. After removal of unbound material, a biotin-conjugated antibody specific for CLPS is added to the wells. After washing, streptavidin-conjugated horseradish peroxidase (HRP) is added to the wells. After washing to remove unbound avidin-enzyme reagent, a substrate solution is added to the wells, and the color is developed proportionally to the amount of CLPS bound in the initial step. The color development is stopped, and the intensity of the color is measured.
Background:
CLPS is a cofactor for pancreatic lipase, allowing the lipase to anchor itself to the lipid-water interface, and can counteract the inhibitory effect of intestinal bile acids on pancreatic lipase activity.
Synonyms:
Pancreatic colipase
Applications:
Pig colipase (CLPS) ELISA kit (0.156-10 ng/mL) is used to quantify CLPS in serum, plasma, tissue homogenates, and other biological fluid samples of pig, providing data to support a wide range of studies.
Research Area:
Lipid metabolism; Obesity research
Specification
Sample Type:
Serum; Plasma; Tissue homogenates; Other biological fluids
Detection Range:
0.156-10 ng/mL
Sensitivity:
0.1 ng/mL
Precision (Intra-assay):
CV<8%
Precision (Inter-assay):
CV<12%
Cross-reactivity:
No significant cross-reactivity or interference was observed.
Storage:
Store at 2-8°C.
