Mouse urocortin 2 (UCN2) ELISA kit (0.78-50 ng/mL)

Cat. No.: 0126WXX-2030

Assay Type: Quantitative sandwich ELISA

Target Species: Mouse

Assay Target: UCN2

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Mouse urocortin 2 (UCN2) ELISA kit (0.78-50 ng/mL)

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Product Overview

Description: Mouse urocortin 2 (UCN2) ELISA kit (0.78-50 ng/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of UCN2 in mouse samples with a range of 0.78-50 ng/mL and a minimum detectable dose (sensitivity) of 0.31 ng/mL. The kit is highly sensitive and easy to use.

Assay Principle: The test principle applied in this kit is the sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to UCN2. Standards or samples are added to the appropriate microtiter plate wells along with a biotin-conjugated antibody specific for UCN2. Next, avidin conjugated to HRP is added to each microplate well and incubated. After the TMB substrate solution is added, only those wells containing UCN2, the biotin-conjugated antibody, and the enzyme-conjugated avidin will exhibit a colorimetric change. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution, and the color shift is measured spectrophotometrically at a wavelength of 450 nm ± 10 nm. The final concentration of UCN2 in the samples is determined by mapping the O.D. values of the specimens against the established standard curve.

Background: Urocortin 2 (UCN2) is a secreted peptide of the sauvagine/corticotropin-releasing factor/urotensin I family that binds corticotropin-releasing hormone receptor 2 and other G-protein-coupled receptors. UCN2 regulates physiological processes such as food intake, gastric motility, cAMP signaling, and cellular responses to nutrient levels and stress.

Synonyms: URP; SRP; UCN-II; UCNII; Stresscopin-related peptide; Urocortin-related peptide

Applications: Mouse urocortin 2 (UCN2) ELISA kit (0.78-50 ng/mL) is used to quantify UCN2 in serum, plasma, tissue homogenates, cell lysates, cell culture supernatants, and other biological fluids of mice, providing data to support research in a wide range of areas, including endocrinology, obesity, and others.

Research Area: Endocrinology; Obesity

Specification

Sample Type: Serum; Plasma; Tissue homogenates; Cell lysates; Cell culture supernatants; Other biological fluids

Detection Range: 0.78-50 ng/mL

Sensitivity: 0.31 ng/mL

Precision (Intra-assay): CV<10%

Precision (Inter-assay): CV<12%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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