Mouse pro-opiomelanocortin (POMC) ELISA kit-Quantitative sandwich

Cat. No.: 0126WXX-1439

Assay Type: Quantitative sandwich ELISA

Target Species: Mouse

Assay Target: POMC

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Mouse pro-opiomelanocortin (POMC) ELISA kit-Quantitative sandwich

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Product Overview

Description: Mouse pro-opiomelanocortin (POMC) ELISA kit-Quantitative sandwich is an ELISA-based in vitro research tool designed specifically for the quantitative detection of POMC in mouse samples with a range of 0.156-10 ng/mL and a minimum detectable dose (sensitivity) of <0.05 ng/mL. The kit is highly sensitive and easy to use.

Assay Principle: This experiment utilizes a typical double-sandwich ELISA technique. The microplate is pre-coated with a mouse POMC monoclonal antibody, and the detection antibody is a biotin-labeled polyclonal antibody. Samples and the biotinylated antibody are added to the wells and washed with PBS or TBS, followed by the addition of avidin-peroxidase conjugates. After a thorough wash, the TMB substrate is used for visualization. Under peroxidase catalysis, the TMB turns blue and finally yellow upon the addition of acid. The color depth is positively correlated with the concentration of the target factors in the sample.

Background: POMC, a highly complex precursor polypeptide belonging to the POMC family and encoded at chromosomal location 2p23.3, serves as a central hub in the endocrine regulation of energy homeostasis and systemic metabolism. Synthesized as a secreted protein that undergoes extensive peptide hormone processing, POMC is vital for the neuropeptide signaling pathways that govern appetite regulation, glucose homeostasis, and blood pressure. Its biological significance is underscored by its role in producing ACTH to stimulate adrenal cortisol release, as well as its essential binding activities at melanocortin receptors (MC3R and MC4R) to modulate feeding behavior. Crucially, genetic defects in POMC are directly linked to Pro-opiomelanocortin Deficiency (POMCD) and a heightened susceptibility to early-onset obesity—a condition defined by the excessive accumulation of body fat beyond physiological requirements. Beyond its primary metabolic functions, POMC influences diverse processes ranging from cellular pigmentation and neuropeptide transduction to the negative regulation of tumor necrosis factor production, highlighting its status as a pivotal molecular driver in the complex pathology of metabolic disorders.

Synonyms: POC; NPP; ProOMC; ACTH; Pro-OMC; Pro opiomelanocortin; Corticotropin-lipotropin; Corticotropin; Adrenocorticotropic hormone; Proopiomelanocortin

Applications: Mouse pro-opiomelanocortin (POMC) ELISA kit-Quantitative sandwich is used to quantify POMC in serum, plasma, cell culture supernatant of mouse, providing data to support research in a wide range of areas, including endocrinology, obesity, and others.

Research Area: Endocrinology; Obesity

Specification

Sample Type: Mouse serum; Plasma; Cell culture supernatant

Detection Range: 0.156-10 ng/mL

Sensitivity: <0.05 ng/mL

Precision (Intra-assay): CV≤8%

Precision (Inter-assay): CV≤12%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Recovery: 70-110%

Storage: Store at 2-8°C.

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