Mouse pro-opiomelanocortin (POMC) ELISA kit (15.6-1000 pg/mL)

Cat. No.: 0126WXX-1444

Assay Type: Quantitative competitive ELISA

Target Species: Mouse

Assay Target: POMC

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Mouse pro-opiomelanocortin (POMC) ELISA kit (15.6-1000 pg/mL)

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Product Overview

Description: Mouse pro-opiomelanocortin (POMC) ELISA kit (15.6-1000 pg/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of POMC in mouse samples with a range of 15.6-1000 pg/mL and a minimum detectable dose (sensitivity) of <7.8 pg/mL. The kit is highly sensitive and easy to use.

Assay Principle: This ELISA is based on the competitive binding enzyme immunoassay technique. The provided microtiter plate is pre-coated with an antibody specific to the target antigen. During the assay, the target antigen in the sample or standard competes with a fixed amount of biotin-labeled target antigen for binding sites on the immobilized antibody. Following a wash step to remove excess conjugate and unbound substances, Avidin-conjugated HRP is added to each well and incubated. Subsequently, a TMB substrate solution is introduced. The reaction is terminated with a sulphuric acid solution, and the absorbance is measured at 450 nm ± 2 nm. The target concentration is determined by comparing the sample O.D. to the standard curve.

Background: POMC, a highly complex precursor polypeptide belonging to the POMC family and encoded at chromosomal location 2p23.3, serves as a central hub in the endocrine regulation of energy homeostasis and systemic metabolism. Synthesized as a secreted protein that undergoes extensive peptide hormone processing, POMC is vital for the neuropeptide signaling pathways that govern appetite regulation, glucose homeostasis, and blood pressure. Its biological significance is underscored by its role in producing ACTH to stimulate adrenal cortisol release, as well as its essential binding activities at melanocortin receptors (MC3R and MC4R) to modulate feeding behavior. Crucially, genetic defects in POMC are directly linked to Pro-opiomelanocortin Deficiency (POMCD) and a heightened susceptibility to early-onset obesity—a condition defined by the excessive accumulation of body fat beyond physiological requirements. Beyond its primary metabolic functions, POMC influences diverse processes ranging from cellular pigmentation and neuropeptide transduction to the negative regulation of tumor necrosis factor production, highlighting its status as a pivotal molecular driver in the complex pathology of metabolic disorders.

Synonyms: POC; NPP; ProOMC; ACTH; Pro-OMC; Pro opiomelanocortin; Corticotropin-lipotropin; Corticotropin; Adrenocorticotropic hormone; Proopiomelanocortin

Applications: Mouse pro-opiomelanocortin (POMC) ELISA kit (15.6-1000 pg/mL) is used to quantify POMC in serum, plasma, tissue homogenates, cell culture supernatant, and other biological fluids of mouse, providing data to support research in a wide range of areas, including endocrinology, obesity, and others.

Research Area: Endocrinology; Obesity

Specification

Sample Type: Serum; Plasma; Tissue homogenates; Cell culture supernatants; Other biological fluids

Detection Range: 15.6-1000 pg/mL

Sensitivity: <7.8 pg/mL

Precision (Intra-assay): CV≤6.3%

Precision (Inter-assay): CV≤8.2%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at -20°C.

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