Mouse neurotrophic tyrosine kinase receptor type 2 (NTRK2) ELISA kit (0.156-10 ng/mL)

Cat. No.: 0126WXX-1182

Assay Type: Quantitative sandwich ELISA

Target Species: Mouse

Assay Target: NTRK2

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Mouse neurotrophic tyrosine kinase receptor type 2 (NTRK2) ELISA kit (0.156-10 ng/mL)

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Product Overview

Description: Mouse neurotrophic tyrosine kinase receptor type 2 (NTRK2) ELISA kit (0.156-10 ng/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of NTRK2 in mice samples with a range of 0.156-10 ng/mL and a minimum detectable dose (sensitivity) of 0.094 ng/mL. The kit is highly sensitive and easy to use.

Assay Principle: This kit is based on sandwich ELISA technology. An anti-mouse NTRK2 antibody is pre-coated onto 96-well plates, and a biotin-conjugated anti-mouse NTRK2 antibody is utilized for detection. Standards, test samples, and the biotinylated detection antibody are added to the wells in succession, followed by a wash step with wash buffer. HRP-streptavidin is then added, and any unbound conjugates are washed away. TMB substrates are applied to visualize the HRP enzymatic reaction, where TMB is catalyzed by HRP to generate a blue product that turns yellow after adding the acidic stop solution. The density of the yellow color is proportional to the amount of mouse NTRK2 captured in the wells. The O.D. absorbance is read at 450 nm using a microplate reader to determine the final concentration of mouse NTRK2.

Background: NTRK2, also known as TrkB, is a high-affinity receptor for brain-derived neurotrophic factor (BDNF). The BDNF/TrkB signaling pathway is crucial for neuronal survival, differentiation, and plasticity in the central nervous system (CNS). The link between NTRK2 and obesity research primarily manifests in the CNS regulation of appetite: BDNF serves as a key anorexigenic (appetite-suppressing) signal. Defects in NTRK2 expression and function in the hypothalamus weaken BDNF signaling, leading to overeating, reduced energy expenditure, and severe obesity. Consequently, NTRK2 serves as a key signaling integration point in central obesity regulation pathways (acting synergistically with the MC pathway, leptin signaling, etc.).

Synonyms: OBHD; TRKB; DEE58; trk-B; EIEE58; GP145-TrkB; EC 2.7.10.1; Neurotrophic tyrosine kinase, receptor, type 2; BDNF-Tropomyosine receptor kinase B

EC NO.: 2.7.10.1

Applications: Mouse neurotrophic tyrosine kinase receptor type 2 (NTRK2) ELISA kit (0.156-10 ng/mL) is used to quantify NTRK2 in serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples of mouse, providing data to support research in a wide range of areas, including signal transduction, neurodevelopment, obesity, etc.

Research Area: Signal transduction; Neurodevelopment; Obesity

Specification

Sample Type: Serum; Plasma; Cell culture supernatant; Cell or tissue lysate; Other liquid samples

Detection Range: 0.156-10 ng/mL

Sensitivity: 0.094 ng/mL

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at -20°C (microplate, standard) and 2-8°C (other reagents).

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