Human estrogen receptor (ER) ELISA kit

Cat. No.: OB0625WXX-696

Assay Type: Quantitative competitive or sandwich ELISA

Target Species: Human

Assay Target: ER

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Product Overview

Description: Human estrogen receptor (ER) ELISA kit is an ELISA-based in vitro research tool designed specifically for the quantitative detection of ER in human with a specificity of 1.0 pg/mL.

Assay Principle: The microtiter plate has been pre-coated with a monoclonal antibody specific for ER. Standards or samples are then added to the wells of the microtiter plate, and if ER is present, the ER will bind to the wells pre-coated with the antibody. To quantify the amount of ER in the sample, a standard preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody is added to each well to “clamp” the ER immobilized on the microtiter plate. The microtiter plate is incubated, and then the wells are thoroughly washed to remove all unbound components. The substrate solution is then added to each well. The enzyme (HRP) and substrate react during a brief incubation period. Only wells containing antibodies conjugated to ER and enzyme will show a color change. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution, and the color change is measured spectrophotometrically at 450 nm. A standard curve is plotted between color intensity (O.D.) and standard concentration. The concentration of ER in each sample is deduced from this standard curve.

Background: ER is a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. Estrogens and their receptors play roles in sexual development, reproductive functions, bone, and other tissues. ERs are associated with various pathological processes.

Synonyms: Estradiol receptor; Oestrogen receptor; Receptors, Estrogen

Applications: Human estrogen receptor (ER) ELISA kit is an important tool for the quantitative detection of ER in various biological samples (serum, plasma, cell culture supernatants, body fluid, tissue homogenate) of humans.

Research Area: Endocrinology

Specification

Sample Type: Serum; Plasma; Cell culture supernatants; Body fluid; Tissue homogenate

Sensitivity: 1.0 pg/mL

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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