Mouse insulin receptor substrate 1 (IRS-1) ELISA kit-Quantitative competitive

Cat. No.: 0126WXX-706

Assay Type: Quantitative competitive ELISA

Target Species: Mouse

Assay Target: IRS-1

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Mouse insulin receptor substrate 1 (IRS-1) ELISA kit-Quantitative competitive

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Product Overview

Description: Mouse insulin receptor substrate 1 (IRS-1) ELISA kit-Quantitative competitive is an ELISA-based in vitro research tool designed specifically for the quantitative detection of IRS-1 in mice samples with a minimum detectable dose (sensitivity) of 0.1 ng/mL. The kit is highly sensitive and easy to use.

Assay Principle: This IRS1 ELISA kit utilizes a competitive enzyme immunoassay format featuring a monoclonal anti-IRS1 antibody and an IRS1-HRP conjugate. Samples and assay buffers are incubated with the HRP-labeled conjugate in the pre-sensitized plate for sixty minutes. Because the binding sites on the monoclonal antibody are finite, the IRS1 in the sample competes with the conjugate for these sites. After five wash cycles, an HRP substrate is introduced, forming a blue product that turns yellow upon acidification. The signal intensity at 450 nm is inversely proportional to the IRS1 concentration. Final values are interpolated from a standard curve relating O.D. to the concentration of standards.

Background: IRS-1 is a key adaptor protein that plays a central role in insulin signaling. When insulin binds to its receptor on the cell surface, the receptor is activated and phosphorylates tyrosine residues on IRS-1. This process transforms IRS-1 into a signaling “relay station,” enabling it to bind with multiple downstream signaling proteins such as phosphoinositide 3-kinase (PI3K). IRS-1 transmits insulin signals from the cell membrane into the cell interior, thereby initiating a series of vital biological responses, including promoting glucose transport and glycogen synthesis. Studying the function and defects of IRS-1 is crucial for understanding the pathological processes underlying obesity.

Synonyms: IRS1

Applications: Mouse insulin receptor substrate 1 (IRS-1) ELISA kit-Quantitative competitive is used to quantify IRS-1 in serum, plasma, cell culture supernatants, body fluid, tissue homogenate of mouse, providing data to support research in a wide range of areas, including endocrinology, obesity, etc.

Research Area: Endocrinology; Obesity

Specification

Sample Type: Serum; Plasma; Cell culture supernatants; Body fluid; Tissue homogenate

Sensitivity: 0.1 ng/mL

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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