Mouse insulin (INS) ELISA kit (78.1-5000 pg/mL)

Cat. No.: 0126WXX-597

Assay Type: Quantitative sandwich ELISA

Target Species: Mouse

Assay Target: INS

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Mouse insulin (INS) ELISA kit (78.1-5000 pg/mL)

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Product Overview

Description: Mouse insulin (INS) ELISA kit (78.1-5000 pg/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of INS in mouse samples with a range of 78.1-5000 pg/mL and a minimum detectable dose (sensitivity) of 30.2 pg/mL. The kit is highly sensitive and easy to use.

Assay Principle: This kit utilizes a sandwich immunoassay format where the microtiter plate is pre-coated with an INS-specific antibody. Standards or samples are added to the wells alongside a biotin-conjugated detection antibody and avidin-HRP. Following incubation and TMB substrate addition, wells containing the INS complex exhibit a color change. The reaction is terminated with sulphuric acid, and the O.D. is measured at 450 ± 10 nm. The concentration of insulin is determined by comparing sample O.D. values to a plotted standard curve.

Background: INS is a hormone composed of an A chain and a B chain linked by two disulfide bonds. Secreted by the pancreas, it functions as a key regulator of metabolism within the body. Its primary role is to lower blood glucose levels by promoting glucose uptake into cells. INS is fundamentally linked to obesity through its role in energy metabolism and its relationship with insulin resistance. By targeting the insulin receptor signaling pathway and the diverse metabolic processes it regulates, researchers can develop novel anti-obesity therapies.

Synonyms: Pancreatic insulin

Applications: Mouse insulin (INS) ELISA kit (78.1-5000 pg/mL) is used to quantify INS in serum, plasma, cell culture supernatants, and other biological fluids of mouse, providing data to support research in a wide range of areas, including metabolic pathway, endocrinology, hormone metabolism, obesity, etc.

Research Area: Metabolic pathway; Endocrinology; Hormone metabolism; Obesity

Specification

Sample Type: Serum; Plasma; Cell culture supernatants; Other biological fluids

Detection Range: 78.1-5000 pg/mL

Sensitivity: 30.2 pg/mL

Precision (Intra-assay): CV<10%

Precision (Inter-assay): CV<12%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 4°C (TMB substrate, wash buffer, stop solution) and -20°C (others).

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