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Mouse cocaine- and amphetamine-regulated transcript (CART) prepropeptide ELISA kit (0.156-10 ng/mL, 0.078 ng/mL)

Cat. No.: OB0625WXX-264
Assay Type: Quantitative sandwich ELISA
Target Species: Mouse
Assay Target: CART
Size:
Mouse cocaine- and amphetamine-regulated transcript (CART) prepropeptide ELISA kit (0.156-10 ng/mL, 0.078 ng/mL)
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Product Overview

Description: Mouse cocaine- and amphetamine-regulated transcript (CART) prepropeptide ELISA kit (0.156-10 ng/mL, 0.078 ng/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of CART in mouse with a range of 0.156-10 ng/mL and a specificity of 0.078 ng/mL.
Assay Principle: Standards and samples are pipetted into the wells, and any CART present is bound by the immobilized antibody. After removing unbound material, a biotin-conjugated antibody specific for CART is added to the wells. After washing, streptavidin-conjugated horseradish peroxidase (HRP) is added to the wells. After washing to remove unbound avidin-enzyme reagent, a substrate solution is added to the wells, and the color is developed proportionally to the amount of CART bound in the first step. The color development is stopped, and the intensity of the color is measured.
Background: CART is processed by prohormone/proprotein convertases to produce smaller, biologically active peptides and is considered to be involved in the regulation of appetite and stress. Its function is closely related to the actions of Leptin and Neuropeptide Y. Mutations in the gene are associated with obesity susceptibility.
Synonyms: CARTPT
Applications: Mouse cocaine- and amphetamine-regulated transcript (CART) prepropeptide ELISA kit (0.156-10 ng/mL, 0.078 ng/mL) is used to quantify CART in serum, plasma, and other biological fluid samples of mouse, providing data to support a wide range of studies.
Research Area: Appetite regulation; Signal regulation; Obesity research

Specification

Sample Type: Serum; Plasma; Other biological fluids
Detection Range: 0.156-10 ng/mL
Sensitivity: 0.078 ng/mL
Precision (Intra-assay): CV<8%
Precision (Inter-assay): CV<12%
Cross-reactivity: No significant cross-reactivity or interference was observed.
Storage: Store at 2-8°C.

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