Chicken glucagon (GCG) ELISA kit (1.563-100 pg/mL)

Cat. No.: 0126WXX-52

Assay Type: Quantitative competitive ELISA

Target Species: Chicken

Assay Target: GCG

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Chicken glucagon (GCG) ELISA kit (1.563-100 pg/mL)

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Product Overview

Description: Chicken glucagon (GCG) ELISA kit (1.563-100 pg/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of GCG in chicken samples with a range of 1.563-100 pg/mL and a minimum detectable dose (sensitivity) of 0.938 pg/mL. The kit is highly sensitive and easy to use.

Assay Principle: This kit is based on a competitive ELISA method, where the final signal intensity is inversely proportional to the target antigen concentration. The microplate is pre-coated with the target antigen. During the reaction, the antigen in the standard or sample competes with the immobilized antigen for limited binding sites on a biotin-labeled detection antibody. After washing, HRP-streptavidin is added to bind to the biotinylated antibody. Subsequently, a TMB substrate solution is added, and the reaction is halted with sulfuric acid. The resulting color intensity is measured spectrophotometrically at 450 nm and compared to a standard curve.

Background: Glucagon is a hormone secreted by A cells of the islets of Langerhans, playing a pivotal role in glucose metabolism and homeostasis. Its primary function is to regulate blood glucose levels by promoting gluconeogenesis and inhibiting glycolysis. The dynamic equilibrium between glucagon and insulin is crucial for maintaining healthy metabolism. The detection of glucagon and analysis of its associated pathways provide key insights into understanding and treating obesity.

Synonyms: GLP1; GLP2; GRPP; Glicentin-related polypeptide; Glucagen; Oxyntomodulin; Incretin hormone; GC

Applications: Chicken glucagon (GCG) ELISA kit (1.563-100 pg/mL) is used to quantify GCG in serum, plasma, cell culture supernatant, cell, tissue lysate, and other liquid samples of chicken, providing data to support research in a wide range of areas, including endocrinology, obesity, metabolism, and others.

Research Area: Endocrinology; Obesity; Metabolism