Mouse cathepsin S (CTSS) ELISA kit

Cat. No.: OB0625WXX-536

Assay Type: Quantitative ELISA

Target Species: Mouse

Assay Target: CTSS

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Product Overview

Description: Mouse cathepsin S (CTSS) ELISA kit is an ELISA-based in vitro research tool designed specifically for the quantitative detection of CTSS in mouse. The kit is highly specific, sensitive, and easy to use.

Assay Principle: The microtiter plate provided in this kit is pre-coated with an antibody specific for the target antigen. Standards or samples are then added to the appropriate microtiter plate wells along with a biotin-conjugated antibody preparation specific for the target antigen, and avidin conjugated to horseradish peroxidase (HRP) is then added to each microtiter plate well and incubated. TMB substrate solution is then added to each well. Only wells containing the target antigen, biotin-conjugated antibody, and enzyme-conjugated avidin will show a color change. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution, and the color change is measured spectrophotometrically at 450 nm ± 2 nm. The concentration of the target antigen in the sample is then determined by comparing the OD of the sample to the standard curve.

Background: CTSS is an important member of the cysteine protease family. As a potent mammalian elastase, it can degrade various extracellular components, including fibronectin, laminin, elastin, and collagen. CTSS is abnormally expressed in various disease states, such as obesity and atherosclerosis, which makes it a biomarker and a potential therapeutic target. Studies have found that CTSS inhibitors may be a possible strategy for treating obesity.

Synonyms: CTS-S; EC 3.4.22.27

EC NO.: 3.4.22.27

Applications: Mouse cathepsin S (CTSS) ELISA kit is an important tool for the quantitative detection of CTSS in various biological samples (serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids) of mice.

Research Area: Obesity research; Immune response; Signaling pathway; Protein degradation

Specification

Sample Type: Serum; Plasma; Tissue homogenates; Cell culture supernates; Other biological fluids

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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