Mouse beta-klotho (KLB) ELISA kit (31.25-2000 pg/mL)

Cat. No.: 0126WXX-749

Assay Type: Quantitative sandwich ELISA

Target Species: Mouse

Assay Target: KLB

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Mouse beta-klotho (KLB) ELISA kit (31.25-2000 pg/mL)

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Product Overview

Description: Mouse beta-klotho (KLB) ELISA kit (31.25-2000 pg/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of KLB in mice samples with a range of 31.25-2000 pg/mL and a minimum detectable dose (sensitivity) of <7.8 pg/mL. The kit is highly sensitive and easy to use.

Assay Principle: This assay utilizes a quantitative sandwich enzyme immunoassay format. An antibody specific for KLB is pre-immobilized on a microplate. Standards and samples are pipetted into the wells, where KLB is captured by the solid-phase antibody. After washing away unbound substances, an HRP-conjugated antibody specific for KLB is added to the wells. Following a wash to remove any unbound reagent, a substrate solution is introduced. Color develops in direct proportion to the amount of KLB bound. The enzymatic reaction is stopped, and the intensity of the color is recorded.

Background: KLB is a membrane protein serving as an essential co-receptor for fibroblast growth factor (FGF) signaling, specifically mediating the signaling pathways of FGF19 (intestinal origin), FGF21 (hepatic/adipose tissue origin), and FGF15. FGF21 is a crucial metabolic hormone that markedly improves insulin sensitivity, promotes energy expenditure, and reduces body weight, with KLB serving as the essential mediator for cellular reception of FGF21 signaling. Consequently, KLB function and expression are pivotal for investigating FGF21-mediated anti-obesity and anti-diabetic effects.

Synonyms: BetaKlotho; Klotho beta like; Klotho beta-like protein; BKL

Applications: Mouse beta-klotho (KLB) ELISA kit (31.25-2000 pg/mL) is used to quantify KLB in serum, plasma, and tissue homogenates of mouse, providing data to support research in a wide range of areas, including signal transduction, metabolism, obesity, etc.

Research Area: Signal transduction; Metabolism; Obesity

Specification

Sample Type: Serum; Plasma; Tissue homogenates

Detection Range: 31.25-2000 pg/mL

Sensitivity: <7.8 pg/mL

Precision (Intra-assay): CV<8%

Precision (Inter-assay): CV<10%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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