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Mouse adipocyte fatty acid binding protein 4 (FABP4) ELISA kit
Cat. No.:
OB0625WXX-812
Assay Type:
Quantitative sandwich ELISA
Target Species:
Mouse
Assay Target:
FABP4
Size:
Add to basket
Product Overview
Description:
Mouse adipocyte fatty acid binding protein 4 (FABP4) ELISA kit is an ELISA-based in vitro research tool designed specifically for the quantitative detection of FABP4 in mouse with a range of 31.25-2000 pg/mL and a specificity of 7.81 pg/mL.
Assay Principle:
An antibody specific for FABP4 has been pre-coated on a microtiter plate. Standards and samples are pipetted into the wells, and any FABP4 will be bound by the immobilized antibody. After removing unbound material, a biotin-conjugated antibody specific for FABP4 is added to the wells. After washing, avidin-conjugated horseradish peroxidase (HRP) is added to the wells. After washing to remove unbound avidin-enzyme reagent, a substrate solution is added to the wells, and the color develops in proportion to the amount of FABP4 bound in the first step. The color development is stopped and the intensity of the color is measured.
Background:
FABP4 is an intracellular protein highly expressed in adipocytes and macrophages, responsible for binding and transporting fatty acids within these cells.
Synonyms:
Fatty acid binding protein 4, Adipocyte; A-FABP; AFABP; AP2; Adipocyte protein 2
Applications:
Mouse adipocyte fatty acid binding protein 4 (FABP4) ELISA kit is used to quantify FABP4 in serum, plasma, tissue homogenate samples of mouse, providing data to support a wide range of studies.
Research Area:
Lipid metabolism; Obesity research
Specification
Sample Type:
Serum; Plasma; Tissue homogenates
Detection Range:
31.25-2000 pg/mL
Sensitivity:
7.81 pg/mL
Precision (Intra-assay):
CV<8%
Precision (Inter-assay):
CV<10%
Cross-reactivity:
No significant cross-reactivity or interference was observed.
Storage:
Store at 2-8°C.