Monkey apolipoprotein C-III (apo-CIII) ELISA kit (1.56-100 ng/mL)

Cat. No.: OB0625WXX-151

Assay Type: Quantitative sandwich ELISA

Target Species: Monkey

Assay Target: apo-CIII

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Monkey apolipoprotein C-III (apo-CIII) ELISA kit (1.56-100 ng/mL)

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Product Overview

Description: Monkey apolipoprotein C-III (apo-CIII) ELISA kit (1.56-100 ng/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of apo-CIII in monkey with a range of 1.56-100 ng/mL and a specificity of 0.94 ng/mL.

Assay Principle: The micro ELISA plates in this kit are pre-coated with an antibody specific to monkey Apo-CIII. Standards or samples are added to the wells of the micro ELISA plate to bind to the specific antibody. A biotinylated detection antibody specific for monkey Apo-CIII and avidin-horseradish peroxidase (HRP) conjugate are then added sequentially to each microplate well and incubated. The free components are washed away. Substrate solution is added to each well. Only wells containing Monkey Apo-CIII, biotinylated detection antibody, and avidin-HRP conjugate will appear blue. Upon addition of the termination solution, the enzyme-substrate reaction terminates, and the color turns yellow. Optical density (OD) is measured spectrophotometrically at 450 nm ± 2 nm. The OD value is proportional to the concentration of monkey Apo-CIII. The concentration of monkey Apo-CIII in the sample can be calculated by comparing the OD value of the sample with the standard curve.

Background: Apolipoprotein C-III is secreted by the liver and small intestine. It is a very low-density lipoprotein (VLDL) protein that inhibits lipoprotein lipase and hepatic lipase. Studies have found that it plays a key role in the metabolism of triglyceride-rich lipoproteins (TRLs).

Synonyms: ApoC-III ; ApoCIII; Apo C-III; Apo C III; Apolipoprotein CIII; APOC3

Applications: Monkey apolipoprotein C-III (apo-CIII) ELISA kit (1.56-100 ng/mL) is used to quantify apo-CIII in serum, plasma, and other biological fluid samples of monkey, providing data to support a wide range of studies.

Research Area: Lipid metabolism; Fatty acid biosynthesis; Obesity research

Specification

Sample Type: Serum; Plasma; Other biological fluids

Detection Range: 1.56-100 ng/mL

Sensitivity: 0.94 ng/mL

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 4°C.

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