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LipoKnoxa™ Human TCF7L2 shRNA Ad5 Particle (Silencing)
Cat. No.:
V0126XX85
Species:
Human
Target Gene:
TCF7L2
Vector System:
Adenovirus
Modulation Type:
Silencing (shRNA)
SPECIFIC INQUIRY
Add to basket
| Sub Cat. No. | TargetSeq | Region | Inquiry |
|---|---|---|---|
| V0126XX85-1 | CTCCGCACCCTCCAGATATAT | CDS | Inquiry |
| V0126XX85-2 | CCGAAAGTTTCCGAGACAAAT | CDS | Inquiry |
| V0126XX85-3 | CAAGATGGAGGGCTCTTTAAG | CDS | Inquiry |
| V0126XX85-4 | Other | Inquiry |
Product Overview
Description:
LipoKnoxa™ Human TCF7L2 shRNA Ad5 Particle (Silencing) provides an efficient method to knock down transcription factor 7-Like 2, one of the most significant genetic risk factors for Type 2 Diabetes. This tool assists in investigating the Wnt signaling pathway's role in proglucagon expression and adipogenesis. Our quality system ensures viral potency and sterility, providing a reliable foundation for genetic and physiological studies through comprehensive QC screening.
Production Cell Line:
HEK293
Viral Backbone:
Adenovirus type 5 (dE1/E3)
Promoter:
U6; CMV; EF1α; CAG; UBC
Product Availability:
Produced Upon Order
Specification
Titer Test:
qPCR
Insert Verification:
All viral preparations are validated via Sequencing and PCR to ensure 100% sequence identity and the structural integrity of the vector genomes.
Sterility Test:
This product has been certified sterile following comprehensive microbial growth analysis, confirming the absence of bacterial and fungal contamination.
Mycoplasma Test:
This product was certified negative for mycoplasma contamination following stringent QC analysis, ensuring the absence of all mycoplasmal agents.
Other QC:
Beyond standard protocols, we offer customized knockdown efficiency validation through in vitro and in vivo assessments. This includes precise analysis of mRNA/protein reduction and subsequent biological responses to ensure the functional potency of the shRNA-mediated gene silencing.
Storage:
Upon receipt, viral preparations should be immediately transferred to -80°C for long-term storage to ensure maximum stability and maintain product integrity.
Stability:
This product maintains excellent biological activity for 6–12 months (and up to 2 years in specific cases) when stored continuously at -80°C. Once thawed, the working solution remains stable for 2–3 weeks at 4°C without significant loss of viral potency.
Shipping Condition:
All viral preparations are shipped on dry ice to ensure maximum biological activity and stability during transit.
Handling Notes:
Viral particles are susceptible to temperature fluctuations and freeze-thaw cycles. To preserve functional titers, it is essential to aliquot the vector into low-protein-binding tubes immediately upon first thaw. To ensure experimental success and biological safety, all procedures must be conducted within a certified biosafety cabinet.
Intended Use:
This product is intended for research use only and is not for use in diagnosis or therapeutic applications.
Product Disclaimer:
While our products are committed to excellence through rigorous internal QC inspections, we cannot guarantee specific performance or experimental outcomes due to the inherent complexity of biological systems. Users assume full responsibility for product storage, handling, and strict compliance with all applicable safety protocols, biosafety requirements, and legal regulations during all operational processes.
Target Profile
Gene Name:
TCF7L2
Full Name:
Transcription factor 7 like 2
Gene Symbol:
TCF4; TCF-4
Gene ID:
6934
RefSeq ID-1:
NP_001139746.1
RefSeq ID-2:
NM_001146274.2
Summary:
TCF7L2 is a crucial transcription factor within the Wnt signaling pathway, which is essential for proper organ development and glucose metabolism. It is widely acknowledged as the most significant genetic risk factor for the development of Type 2 diabetes. TCF7L2 influences blood glucose homeostasis by regulating insulin production and secretion. Genetic variants in this locus impair the body's ability to respond to glucose, creating a strong hereditary link to metabolic failure.
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