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LipoKnoxa™ Human NR0B2 shRNA Ad5 Particle (Silencing)
Cat. No.:
V0126XX33
Species:
Human
Target Gene:
NR0B2
Vector System:
Adenovirus
Modulation Type:
Silencing (shRNA)
SPECIFIC INQUIRY
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| Sub Cat. No. | TargetSeq | Region | Inquiry |
|---|---|---|---|
| V0126XX33-1 | CTCTTCTTTCGCCCTATCATT | CDS | Inquiry |
| V0126XX33-2 | CCCAGCATACTCAAGAAGATT | CDS | Inquiry |
| V0126XX33-3 | CACATTGGACTTCCTTGGTTT | 3' UTR | Inquiry |
| V0126XX33-4 | Other | Inquiry |
Product Overview
Description:
LipoKnoxa™ Human NR0B2 shRNA Ad5 Particle (Silencing) facilitates the targeted inhibition of the SHP, an unconventional nuclear receptor that acts as a transcriptional corepressor in glucose and lipid metabolism. Utilizing high-efficiency Ad5 delivery of either U6 or miR30-based shRNA, this tool enables the dissection of metabolic networks driving hepatic steatosis. We ensure experimental safety and data integrity through comprehensive QC, including high-precision functional titering and rigorous sterility and mycoplasma testing.
Production Cell Line:
HEK293
Viral Backbone:
Adenovirus type 5 (dE1/E3)
Promoter:
U6; CMV; EF1α; CAG; UBC
Product Availability:
Produced Upon Order
Specification
Titer Test:
qPCR
Insert Verification:
All viral preparations are validated via Sequencing and PCR to ensure 100% sequence identity and the structural integrity of the vector genomes.
Sterility Test:
This product has been certified sterile following comprehensive microbial growth analysis, confirming the absence of bacterial and fungal contamination.
Mycoplasma Test:
This product was certified negative for mycoplasma contamination following stringent QC analysis, ensuring the absence of all mycoplasmal agents.
Other QC:
Beyond standard protocols, we offer customized knockdown efficiency validation through in vitro and in vivo assessments. This includes precise analysis of mRNA/protein reduction and subsequent biological responses to ensure the functional potency of the shRNA-mediated gene silencing.
Storage:
Upon receipt, viral preparations should be immediately transferred to -80°C for long-term storage to ensure maximum stability and maintain product integrity.
Stability:
This product maintains excellent biological activity for 6–12 months (and up to 2 years in specific cases) when stored continuously at -80°C. Once thawed, the working solution remains stable for 2–3 weeks at 4°C without significant loss of viral potency.
Shipping Condition:
All viral preparations are shipped on dry ice to ensure maximum biological activity and stability during transit.
Handling Notes:
Viral particles are susceptible to temperature fluctuations and freeze-thaw cycles. To preserve functional titers, it is essential to aliquot the vector into low-protein-binding tubes immediately upon first thaw. To ensure experimental success and biological safety, all procedures must be conducted within a certified biosafety cabinet.
Intended Use:
This product is intended for research use only and is not for use in diagnosis or therapeutic applications.
Product Disclaimer:
While our products are committed to excellence through rigorous internal QC inspections, we cannot guarantee specific performance or experimental outcomes due to the inherent complexity of biological systems. Users assume full responsibility for product storage, handling, and strict compliance with all applicable safety protocols, biosafety requirements, and legal regulations during all operational processes.
Target Profile
Gene Name:
NR0B2
Full Name:
Nuclear receptor subfamily 0 group B member 2
Gene Symbol:
SHP; SHP1
Gene ID:
8431
RefSeq ID-1:
NP_068804.1
RefSeq ID-2:
NM_021969.3
Summary:
The NR0B2 gene encodes an atypical orphan nuclear receptor that contains a putative ligand-binding domain but lacks a conventional DNA-binding domain. As a member of the nuclear hormone receptor family, it interacts with retinoid, thyroid, and estrogen receptors, inhibiting their ligand-dependent transcriptional activation via coactivator competition and direct repression. NR0B2 is involved in metabolic regulation, and altered expression or function has been linked to obesity, suggesting a role in controlling energy homeostasis and adiposity.
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