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LipoKnoxa™ Human MFN2 shRNA Ad5 Particle (Silencing)
Cat. No.:
V0126XX81
Species:
Human
Target Gene:
MFN2
Vector System:
Adenovirus
Modulation Type:
Silencing (shRNA)
SPECIFIC INQUIRY
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| Sub Cat. No. | TargetSeq | Region | Inquiry |
|---|---|---|---|
| V0126XX81-1 | GCAGGTTTACTGCGAGGAAAT | CDS | Inquiry |
| V0126XX81-2 | GCTCAGTGCTTCATCCCATTT | 3' UTR | Inquiry |
| V0126XX81-3 | GCACTTTGTCACTGCCAAGAA | CDS | Inquiry |
| V0126XX81-4 | Other | Inquiry |
Product Overview
Description:
LipoKnoxa™ Human MFN2 shRNA Ad5 Particle (Silencing) targets mitofusin-2, a protein essential for mitochondrial fusion and the maintenance of ER-mitochondria contacts. Silencing MFN2 allows for the investigation of mitochondrial dysfunction and its contribution to insulin resistance and obesity. Our platform ensures experimental success via thorough QC validation—including mycoplasma detection and high-sensitivity titer assays—offering researchers a secure and high-performing gene-silencing solution.
Production Cell Line:
HEK293
Viral Backbone:
Adenovirus type 5 (dE1/E3)
Promoter:
U6; CMV; EF1α; CAG; UBC
Product Availability:
Produced Upon Order
Specification
Titer Test:
qPCR
Insert Verification:
All viral preparations are validated via Sequencing and PCR to ensure 100% sequence identity and the structural integrity of the vector genomes.
Sterility Test:
This product has been certified sterile following comprehensive microbial growth analysis, confirming the absence of bacterial and fungal contamination.
Mycoplasma Test:
This product was certified negative for mycoplasma contamination following stringent QC analysis, ensuring the absence of all mycoplasmal agents.
Other QC:
Beyond standard protocols, we offer customized knockdown efficiency validation through in vitro and in vivo assessments. This includes precise analysis of mRNA/protein reduction and subsequent biological responses to ensure the functional potency of the shRNA-mediated gene silencing.
Storage:
Upon receipt, viral preparations should be immediately transferred to -80°C for long-term storage to ensure maximum stability and maintain product integrity.
Stability:
This product maintains excellent biological activity for 6–12 months (and up to 2 years in specific cases) when stored continuously at -80°C. Once thawed, the working solution remains stable for 2–3 weeks at 4°C without significant loss of viral potency.
Shipping Condition:
All viral preparations are shipped on dry ice to ensure maximum biological activity and stability during transit.
Handling Notes:
Viral particles are susceptible to temperature fluctuations and freeze-thaw cycles. To preserve functional titers, it is essential to aliquot the vector into low-protein-binding tubes immediately upon first thaw. To ensure experimental success and biological safety, all procedures must be conducted within a certified biosafety cabinet.
Intended Use:
This product is intended for research use only and is not for use in diagnosis or therapeutic applications.
Product Disclaimer:
While our products are committed to excellence through rigorous internal QC inspections, we cannot guarantee specific performance or experimental outcomes due to the inherent complexity of biological systems. Users assume full responsibility for product storage, handling, and strict compliance with all applicable safety protocols, biosafety requirements, and legal regulations during all operational processes.
Target Profile
Gene Name:
MFN2
Full Name:
Mitofusin 2
Gene Symbol:
HSG; MSL; MARF; CMT2A; CPRP1; CMT2A2; HMSN6A; CMT2A2A; CMT2A2B
Gene ID:
9927
RefSeq ID-1:
NP_001121132.1
RefSeq ID-2:
NM_001127660.2
Summary:
MFN2 encodes a dynamin-like GTPase on the outer mitochondrial membrane that is essential for mitochondrial fusion. This process preserves the integrity of the mitochondrial network and ensures efficient cellular energy production. Beyond mitochondrial dynamics, MFN2 regulates vascular smooth muscle cell proliferation and has been identified as a player in the broader pathophysiology of obesity. While genetic defects are primarily linked to peripheral neuropathies like Charcot-Marie-Tooth disease, their role in metabolic homeostasis is a critical area of study for metabolic dysfunction and stroke.
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