LipoKnoxa™ Human ICAM1 shRNA Ad5 Particle (Silencing)

Cat. No.: V0126XX121

Species: Human

Target Gene: ICAM1

Vector System: Adenovirus

Modulation Type: Silencing (shRNA)

LipoKnoxa™ Human ICAM1 shRNA Ad5 Particle (Silencing)

INQUIRY Datasheet MSDS

Sub Cat. No.	TargetSeq	Region	Inquiry
V0126XX121-1	CGGCTGACGTGTGCAGTAATA	CDS	Inquiry
V0126XX121-2	GCCCGAGCTCAAGTGTCTAAA	CDS	Inquiry
V0126XX121-3	CCTCAGCACGTACCTCTATAA	CDS	Inquiry
V0126XX121-4	Other		Inquiry

Product Overview

Description: LipoKnoxa™ Human ICAM1 shRNA Ad5 Particle (Silencing) provides a specialized solution for silencing ICAM1, a key player in the recruitment of inflammatory cells into adipose tissue. This tool is indispensable for studying the vascular and inflammatory complications of obesity. Supporting flexible reporter gene selection and U6/miR30 shRNA systems, the product is verified through rigorous QC to ensure safety and high-fidelity signaling analysis.

Production Cell Line: HEK293

Viral Backbone: Adenovirus type 5 (dE1/E3)

Promoter: U6; CMV; EF1α; CAG; UBC

Product Availability: Produced Upon Order

Specification

Titer Test: qPCR

Insert Verification: All viral preparations are validated via Sequencing and PCR to ensure 100% sequence identity and the structural integrity of the vector genomes.

Sterility Test: This product has been certified sterile following comprehensive microbial growth analysis, confirming the absence of bacterial and fungal contamination.

Mycoplasma Test: This product was certified negative for mycoplasma contamination following stringent QC analysis, ensuring the absence of all mycoplasmal agents.

Other QC: Beyond standard protocols, we offer customized knockdown efficiency validation through in vitro and in vivo assessments. This includes precise analysis of mRNA/protein reduction and subsequent biological responses to ensure the functional potency of the shRNA-mediated gene silencing.

Storage: Upon receipt, viral preparations should be immediately transferred to -80°C for long-term storage to ensure maximum stability and maintain product integrity.

Stability: This product maintains excellent biological activity for 6–12 months (and up to 2 years in specific cases) when stored continuously at -80°C. Once thawed, the working solution remains stable for 2–3 weeks at 4°C without significant loss of viral potency.

Shipping Condition: All viral preparations are shipped on dry ice to ensure maximum biological activity and stability during transit.

Handling Notes: Viral particles are susceptible to temperature fluctuations and freeze-thaw cycles. To preserve functional titers, it is essential to aliquot the vector into low-protein-binding tubes immediately upon first thaw. To ensure experimental success and biological safety, all procedures must be conducted within a certified biosafety cabinet.

Intended Use: This product is intended for research use only and is not for use in diagnosis or therapeutic applications.

Product Disclaimer: While our products are committed to excellence through rigorous internal QC inspections, we cannot guarantee specific performance or experimental outcomes due to the inherent complexity of biological systems. Users assume full responsibility for product storage, handling, and strict compliance with all applicable safety protocols, biosafety requirements, and legal regulations during all operational processes.

Target Profile

Gene Name: ICAM1

Full Name: Intercellular adhesion molecule 1

Gene Symbol: BB2; CD54; P3.58

Gene ID: 3383

RefSeq ID-1: NP_000192.2

RefSeq ID-2: NM_000201.3

Summary: ICAM1 is a glycoprotein expressed on endothelial and immune cells. It facilitates the adhesion of white blood cells to the blood vessel wall. In obesity, elevated ICAM1 is a sign of vascular inflammation, which links excessive weight to increased cardiovascular risk.

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