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LipoKnoxa™ Human DGAT1 shRNA Ad5 Particle (Silencing)
Cat. No.:
V0126XX86
Species:
Human
Target Gene:
DGAT1
Vector System:
Adenovirus
Modulation Type:
Silencing (shRNA)
SPECIFIC INQUIRY
Add to basket
| Sub Cat. No. | TargetSeq | Region | Inquiry |
|---|---|---|---|
| V0126XX86-1 | TTGAGCAATGCCCGGTTATTT | CDS | Inquiry |
| V0126XX86-2 | CTTGAGCAATGCCCGGTTATT | CDS | Inquiry |
| V0126XX86-3 | CATCGCCTGCAGGATTCTTTA | CDS | Inquiry |
| V0126XX86-4 | Other | Inquiry |
Product Overview
Description:
LipoKnoxa™ Human DGAT1 shRNA Ad5 Particle (Silencing) targets diglyceride acyltransferase 1, the enzyme responsible for the final step of triglyceride synthesis. Utilizing optimized U6 or miR30 shRNA delivery, this tool allows for the exploration of intestinal fat absorption and lipid droplet formation in adipocytes. Each product is backed by comprehensive QC documentation, confirming sequence integrity and the absence of pathogens (mycoplasma/sterility).
Production Cell Line:
HEK293
Viral Backbone:
Adenovirus type 5 (dE1/E3)
Promoter:
U6; CMV; EF1α; CAG; UBC
Product Availability:
Produced Upon Order
Specification
Titer Test:
qPCR
Insert Verification:
All viral preparations are validated via Sequencing and PCR to ensure 100% sequence identity and the structural integrity of the vector genomes.
Sterility Test:
This product has been certified sterile following comprehensive microbial growth analysis, confirming the absence of bacterial and fungal contamination.
Mycoplasma Test:
This product was certified negative for mycoplasma contamination following stringent QC analysis, ensuring the absence of all mycoplasmal agents.
Other QC:
Beyond standard protocols, we offer customized knockdown efficiency validation through in vitro and in vivo assessments. This includes precise analysis of mRNA/protein reduction and subsequent biological responses to ensure the functional potency of the shRNA-mediated gene silencing.
Storage:
Upon receipt, viral preparations should be immediately transferred to -80°C for long-term storage to ensure maximum stability and maintain product integrity.
Stability:
This product maintains excellent biological activity for 6–12 months (and up to 2 years in specific cases) when stored continuously at -80°C. Once thawed, the working solution remains stable for 2–3 weeks at 4°C without significant loss of viral potency.
Shipping Condition:
All viral preparations are shipped on dry ice to ensure maximum biological activity and stability during transit.
Handling Notes:
Viral particles are susceptible to temperature fluctuations and freeze-thaw cycles. To preserve functional titers, it is essential to aliquot the vector into low-protein-binding tubes immediately upon first thaw. To ensure experimental success and biological safety, all procedures must be conducted within a certified biosafety cabinet.
Intended Use:
This product is intended for research use only and is not for use in diagnosis or therapeutic applications.
Product Disclaimer:
While our products are committed to excellence through rigorous internal QC inspections, we cannot guarantee specific performance or experimental outcomes due to the inherent complexity of biological systems. Users assume full responsibility for product storage, handling, and strict compliance with all applicable safety protocols, biosafety requirements, and legal regulations during all operational processes.
Target Profile
Gene Name:
DGAT1
Full Name:
Diacylglycerol O-acyltransferase 1
Gene Symbol:
ARAT; DGAT; ARGP1; DIAR7
Gene ID:
8694
RefSeq ID-1:
NP_036211.2
RefSeq ID-2:
NM_012079.6
Summary:
DGAT1 is a transmembrane enzyme that catalyzes the final, rate-limiting step of triglyceride synthesis. By converting diacylglycerol and fatty acyl-CoA into triacylglycerol (the primary form of stored body fat), this protein dictates the efficiency of lipid storage in adipose tissue. Additionally, it can transfer acyl groups to retinol. Given its role in fat accumulation, DGAT1 activity is a central focus in studies of obesity and the metabolic consequences of excessive triglyceride storage.
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