Human uncoupling protein-1 (UCP1) ELISA kit (31.25-2000 pg/mL)

Cat. No.: 0126WXX-2041

Assay Type: Quantitative sandwich ELISA

Target Species: Human

Assay Target: UCP1

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Human uncoupling protein-1 (UCP1) ELISA kit (31.25-2000 pg/mL)

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Product Overview

Description: Human uncoupling protein-1 (UCP1) ELISA kit (31.25-2000 pg/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of UCP1 in human samples with a range of 31.25-2000 pg/mL and a minimum detectable dose (sensitivity) of <7.81 pg/mL. The kit is highly sensitive and easy to use.

Assay Principle: This assay employs a quantitative sandwich enzyme immunoassay technique. An antibody specific for UCP1 is pre-coated onto a microplate. Standards and samples are pipetted into the wells, where any UCP1 present is captured by the immobilized antibody. After removing unbound substances, a biotin-conjugated antibody specific for UCP1 is added to the wells. Following a wash step, Avidin-conjugated HRP is added. After another wash to remove any unbound Avidin-enzyme reagent, a substrate solution is added; color develops in proportion to the amount of UCP1 bound in the initial step. The color development is stopped, and the intensity is measured.

Background: Uncoupling protein 1 (UCP1) is a mitochondrial transporter that mediates proton leakage across the inner mitochondrial membrane, uncoupling oxidative phosphorylation from ATP synthesis and dissipating energy as heat. UCP1 plays a key role in brown adipose tissue thermogenesis and energy expenditure, contributing to the regulation of whole-body metabolism. Impaired UCP1 function can reduce thermogenic capacity and promote energy storage, linking it to obesity and related metabolic disorders.

Synonyms: SLC25A7; UCP; Thermogenin; Solute carrier family 25 member 7; Uncoupling Protein 1; Mitochondrial; Mitochondrial brown fat uncoupling protein 1

Applications: Human uncoupling protein-1 (UCP1) ELISA kit (31.25-2000 pg/mL) is used to quantify UCP1 in serum, plasma, and tissue homogenates of humans, providing data to support research in a wide range of areas, including signal transduction, obesity, and others.

Research Area: Signal transduction; Obesity

Specification

Sample Type: Serum; Plasma; Tissue homogenates

Detection Range: 31.25-2000 pg/mL

Sensitivity: <7.81 pg/mL

Precision (Intra-assay): CV<10%

Precision (Inter-assay): CV<12%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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