Human uncoupling protein-1 (UCP1) ELISA kit (0.156-10 ng/mL)

Cat. No.: 0126WXX-2040

Assay Type: Quantitative sandwich ELISA

Target Species: Human

Assay Target: UCP1

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Human uncoupling protein-1 (UCP1) ELISA kit (0.156-10 ng/mL)

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Product Overview

Description: Human uncoupling protein-1 (UCP1) ELISA kit (0.156-10 ng/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of UCP1 in human samples with a range of 0.156-10 ng/mL and a minimum detectable dose (sensitivity) of 0.05 ng/mL. The kit is highly sensitive and easy to use.

Assay Principle: This experiment utilizes a typical double-antibody sandwich ELISA technique. The pre-coated antibody is a human UCP1 monoclonal antibody, while the detecting antibody is a biotin-labeled polyclonal antibody. Samples and biotin-labeling antibodies are added to the microplate wells and washed with PBS or TBS. Avidin-peroxidase conjugates are then introduced into the wells. Following a thorough wash of the reactants with PBS or TBS, TMB substrate is added for color development. The TMB substrate turns blue through peroxidase activity and eventually turns yellow under acidic conditions. The intensity of the color is positively correlated with the testing factors present in the samples.

Background: Uncoupling protein 1 (UCP1) is a mitochondrial transporter that mediates proton leakage across the inner mitochondrial membrane, uncoupling oxidative phosphorylation from ATP synthesis and dissipating energy as heat. UCP1 plays a key role in brown adipose tissue thermogenesis and energy expenditure, contributing to the regulation of whole-body metabolism. Impaired UCP1 function can reduce thermogenic capacity and promote energy storage, linking it to obesity and related metabolic disorders.

Synonyms: SLC25A7; UCP; Thermogenin; Solute carrier family 25 member 7; Uncoupling Protein 1; Mitochondrial; Mitochondrial brown fat uncoupling protein 1

Applications: Human uncoupling protein-1 (UCP1) ELISA kit (0.156-10 ng/mL) is used to quantify UCP1 in serum, plasma, cell culture supernatant, and other human biological fluids, providing data to support research in a wide range of areas, including signal transduction, obesity, and others.

Research Area: Signal transduction; Obesity