Human tumor necrosis factor receptor superfamily member 1B (TNF-RII) ELISA kit (0.625-40 ng/mL)

Cat. No.: 0126WXX-1921

Assay Type: Quantitative ELISA

Target Species: Human

Assay Target: TNF-RII

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Human tumor necrosis factor receptor superfamily member 1B (TNF-RII) ELISA kit (0.625-40 ng/mL)

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Product Overview

Description: Human tumor necrosis factor receptor superfamily member 1B (TNF-RII) ELISA kit (0.625-40 ng/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of TNF-RII in human samples with a range of 0.625-40 ng/mL and a minimum detectable dose (sensitivity) of 0.156 ng/mL. The kit is highly sensitive and easy to use.

Assay Principle: The microtiter plate provided in this kit is pre-coated with an antibody specific to TNF-RII. Standards or samples are added to the appropriate microtiter plate wells along with a biotin-conjugated polyclonal detection antibody specific for TNF-RII. Subsequently, avidin conjugated to HRP is added and incubated. A TMB substrate solution is then introduced. Color development occurs exclusively in wells containing the TNF-RII-biotin-avidin complex. The enzymatic reaction is halted with a sulphuric acid solution, and the color change is recorded at 450 nm ± 2 nm. Final concentrations are determined by comparing the O.D. of the samples to the generated standard curve.

Background: TNF-RII, also known as TNFRSF1B or TNF-R2, is a transmembrane receptor on the cell surface that primarily binds to the pro-inflammatory cytokine tumor necrosis factor TNF-α and plays a crucial role in mediating its biological functions. Unlike TNF-RI, which primarily mediates apoptosis, TNF-RII signaling predominantly activates cell survival and proliferation signals by recruiting the TRAF protein family. In obesity research, TNF-α is a key inflammatory factor released from hypertrophic adipose tissue and is considered one of the primary drivers of insulin resistance. TNF-RII is recognized as mediating most of TNF-α's metabolic effects, particularly in immune cells and endothelial cells. Elevated TNF-RII activity correlates with chronic inflammation, adipose tissue remodeling, and impaired insulin signaling, positioning it as a potential target linking obesity-induced chronic inflammation to metabolic dysfunction.

Synonyms: CD120b antigen; CD120b; Etanercept; p75 TNF receptor; p75TBPII; p75TNFR; Soluble TNFR1B variant 1; TNF RII; TNFBRp80 TNF-alpha receptor; TNF-R2; TNFR2TNFR1B; TNF-R75; TNFR80; TNFRII; TNF-R-II; TNFR-II; TNFRSF1B; Tumor necrosis factor beta receptor; Tumor necrosis factor binding protein 2; Tumor necrosis factor receptor 2; Tumor necrosis factor receptor superfamily; member 1B; Tumor necrosis factor receptor type II

Applications: Human tumor necrosis factor receptor superfamily member 1B (TNF-RII) ELISA kit (0.625-40 ng/mL) is used to quantify TNF-RII in serum, plasma, and cell culture supernatants of human, providing data to support research in a wide range of areas, including inflammation and immunology, metabolism, obesity, and others.

Research Area: Inflammation and immunology; Metabolism; Obesity

Specification

Sample Type: Serum; Plasma; Cell culture supernatants

Detection Range: 0.625-40 ng/mL

Sensitivity: 0.156 ng/mL

Precision (Intra-assay): CV<8%

Precision (Inter-assay): CV<10%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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