Human serine palmitoyltransferase; long chain base subunit 3 (SPTLC3) ELISA kit-Quantitative sandwich

Cat. No.: 0126WXX-1838

Assay Type: Quantitative sandwich ELISA

Target Species: Human

Assay Target: SPTLC3

Size:

Human serine palmitoyltransferase; long chain base subunit 3 (SPTLC3) ELISA kit-Quantitative sandwich

INQUIRY Datasheet MSDS

Add to basket

Product Overview

Description: Human serine palmitoyltransferase; long chain base subunit 3 (SPTLC3) ELISA kit-Quantitative sandwich is an ELISA-based in vitro research tool designed specifically for the quantitative detection of SPTLC3 in human samples with a range of 0.25-8 ng/mL and a minimum detectable dose (sensitivity) of 0.1 ng/mL. The kit is highly sensitive and easy to use.

Assay Principle: The ELISA analytical biochemical technique is based on SPTLC3 antibody-SPTLC3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SPTLC3 antigen targets in samples.

Background: Serine palmitoyltransferase long chain base subunit 3 (SPTLC3) is a key enzyme subunit of serine palmitoyltransferase, the rate-limiting enzyme in sphingolipid biosynthesis. SPTLC3 catalyzes the condensation of serine with fatty acyl-CoA to produce long-chain base sphingoid intermediates, which are essential for the generation of complex sphingolipids involved in cell membrane structure and signaling. Proper SPTLC3 activity is critical for lipid homeostasis, while dysregulation can lead to altered sphingolipid metabolism, contributing to impaired adipocyte function, lipid accumulation, and the development of obesity and obesity-related metabolic disorders.

Synonyms: SPTLC2L; Long chain base biosynthesis protein 3; SPT 3; LCB 3; C20orf38; Aminotransferase 2; Serine-palmitoyl-CoA transferase 3

Applications: Human serine palmitoyltransferase, long chain base subunit 3 (SPTLC3) ELISA kit-Quantitative sandwich is used to quantify SPTLC3 in undiluted original human body fluids, tissue homogenates, secretions, and feces samples of human, providing data to support research in a wide range of areas, including enzyme and kinase, obesity, and others.

Research Area: Enzyme & Kinase; Obesity

Specification

Sample Type: Undiluted original human body fluids; Tissue homogenates; Secretions; Feces samples

Detection Range: 0.25-8 ng/mL

Sensitivity: 0.1 ng/mL

Precision (Intra-assay): CV<15%

Precision (Inter-assay): CV<15%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

Related Products

Inquiry

Inquiry Basket

Delete selected Check Out