Human putative peptide YY3 (PYY3) ELISA kit-Quantitative competitive

Cat. No.: 0126WXX-1692

Assay Type: Quantitative competitive ELISA

Target Species: Human

Assay Target: PYY3

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Human putative peptide YY3 (PYY3) ELISA kit-Quantitative competitive

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Product Overview

Description: Human putative peptide YY3 (PYY3) ELISA kit-Quantitative competitive is an ELISA-based in vitro research tool designed specifically for the quantitative detection of PYY3 in human samples with a range of 12.35-1000 pg/mL and a minimum detectable dose (sensitivity) of 4.82 pg/mL. The kit is highly sensitive and easy to use.

Assay Principle: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to PYY3 is pre-coated onto the microplate. A competitive inhibition reaction is initiated between the biotin-labeled PYY3 and unlabeled PYY3 (from standards or samples) for the limited binding sites on the immobilized antibody. After incubation, unbound conjugates are washed away. Subsequently, avidin conjugated to HRP is added to each well and incubated. The amount of bound HRP conjugate is inversely proportional to the concentration of PYY3 in the sample. Upon the addition of the substrate solution, the intensity of the developed color is inversely proportional to the PYY3 concentration, which is then quantified by comparing the results against a standard curve.

Background: Putative peptide YY3 (PYY3) is a proposed peptide derived from the peptide YY precursor, which belongs to the neuropeptide Y family and is involved in the regulation of appetite and energy balance. Peptide YY–related peptides are primarily secreted by enteroendocrine cells in the gastrointestinal tract and act on Y receptors to modulate food intake and gastrointestinal function. Although the precise biological role of PYY3 remains under investigation, alterations in PYY signaling pathways are known to influence satiety, feeding behavior, and body weight regulation. Dysregulation of PYY-derived peptides is therefore considered relevant to obesity and metabolic disorders associated with impaired appetite control.

Synonyms: PYY-III; Putative peptide YY3

Applications: Human putative peptide YY3 (PYY3) ELISA kit-Quantitative competitive is used to quantify PYY3 in serum, plasma, and other biological fluids of humans, providing data to support research in a wide range of areas, including neuroscience, hepatology, obesity, and others.

Research Area: Neuro science; Hepatology; Obesity

Specification

Sample Type: Serum; Plasma; Other biological fluids

Detection Range: 12.35-1000 pg/mL

Sensitivity: 4.82 pg/mL

Precision (Intra-assay): CV<10%

Precision (Inter-assay): CV<12%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Recovery: 85-104%

Storage: Store at 2-8°C.

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