Human peroxisome proliferator-activated receptor α (PPARα) ELISA kit (0.16-10 ng/mL)

Cat. No.: 0126WXX-1483

Assay Type: Quantitative sandwich ELISA

Target Species: Human

Assay Target: PPARα

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Human peroxisome proliferator-activated receptor α (PPARα) ELISA kit (0.16-10 ng/mL)

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Product Overview

Description: Human peroxisome proliferator-activated receptor α (PPARα) ELISA kit (0.16-10 ng/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of PPARα in human samples with a range of 0.16-10 ng/mL and a minimum detectable dose (sensitivity) of 0.1 ng/mL. The kit is highly sensitive and easy to use.

Assay Principle: This ELISA kit utilizes the sandwich-ELISA principle. The microplate provided is pre-coated with an antibody specific to human PPARα. Standards or samples are added to the wells and combined with the capture antibody. A biotinylated detection antibody and an avidin-HRP conjugate are added successively and incubated. After washing away free components, a substrate solution is added. Only wells containing the human PPARα complex will appear blue, turning yellow upon adding the stop solution. The O.D. is measured at 450 ± 2 nm, and the value is proportional to the concentration of human PPARα.

Background: PPAR-alpha, a member of the NR1 nuclear hormone receptor subfamily located at chromosomal position 22q13.31, serves as a master ligand-activated transcription factor and a cornerstone regulator of lipid homeostasis in obesity research. Primarily localized within the nucleus, its transactivation activity—requiring heterodimerization with RXRA and being antagonized by NR2C2—is triggered by various endogenous ligands such as 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphocholine (16:0/18:1-GPC), satiety-regulating lipids like oleylethanolamide, and exogenous peroxisome proliferators. Beyond its fundamental role in activating the peroxisomal beta-oxidation pathway through genes such as ACOX1 and P450, PPAR-alpha orchestrates a complex physiological network including fatty acid transport, lipoprotein metabolism, and the negative regulation of appetite and glycolysis. Its multifaceted molecular functions—ranging from zinc ion binding to steroid hormone-mediated signaling—integrate critical biological processes such as insulin stimulus response, gluconeogenesis, and circadian rhythm regulation, making it an essential target for understanding and intervening in metabolic disorders and chronic obesity.

Synonyms: Peroxisome proliferator activated receptor alpha; NR1C1; PPAR-A; HPPAR; Nuclear receptor subfamily 1 group C member 1; PPARa; PPARA; PPAR-alpha

Applications: Human peroxisome proliferator-activated receptor α (PPARα) ELISA kit (0.16-10 ng/mL) is used to quantify PPARα in serum, plasma, and other biological fluids of humans, providing data to support research in a wide range of areas, including signal transduction, developmental science, obesity, and others.

Research Area: Signal transduction; Developmental science; Obesity

Specification

Sample Type: Serum; Plasma; Other biological fluids

Detection Range: 0.16-10 ng/mL

Sensitivity: 0.1 ng/mL

Precision (Intra-assay): CV<10%

Precision (Inter-assay): CV<12%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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