Human peptide YY (PYY) ELISA kit-Quantitative sandwich (62.5-4000 pg/mL)

Cat. No.: 0126WXX-1652

Assay Type: Quantitative sandwich ELISA

Target Species: Human

Assay Target: PYY

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Human peptide YY (PYY) ELISA kit-Quantitative sandwich (62.5-4000 pg/mL)

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Product Overview

Description: Human peptide YY (PYY) ELISA kit-Quantitative sandwich (62.5-4000 pg/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of PYY in human samples with a range of 62.5-4000 pg/mL and a minimum detectable dose (sensitivity) of <15.6 pg/mL. The kit is highly sensitive and easy to use.

Assay Principle: This assay employs a quantitative sandwich enzyme immunoassay technique. A microplate is pre-coated with an antibody specific to PYY. Standards and samples are pipetted into the wells, where any PYY present is immobilized by the capture antibody. After removing unbound substances, a biotin-conjugated detection antibody specific for PYY is added. Following a wash, Avidin-conjugated HRP is introduced. After a final wash to remove unbound reagents, a substrate solution is added, and color develops in direct proportion to the amount of PYY bound in the initial step. The reaction is stopped, and the color intensity is measured.

Background: PYY (Peptide YY), a critical gut peptide and member of the NPY family located at chromosomal position 17q21.1, serves as a fundamental regulator in the physiological landscape of obesity and digestive homeostasis. Secreted into the extracellular region as two distinct isoforms through alternative splicing, this neuropeptide hormone exerts its biological influence primarily through G-protein coupled receptor signaling pathways to modulate eating behavior and appetite regulation. Its multifaceted role in the gastrointestinal tract includes the inhibition of exocrine pancreatic secretion, the mediation of vasoconstrictory actions, and the suppression of jejunal and colonic motility, thereby slowing digestion to enhance satiety. Beyond its classical endocrine functions in feeding behavior, PYY is also implicated in broader cellular processes such as cell proliferation and cytoskeleton organization.

Synonyms: Peptide YY-like

Applications: Human peptide YY (PYY) ELISA kit-Quantitative sandwich (62.5-4000 pg/mL) is used to quantify PYY in Serum, Plasma, Urine, and tissue homogenates of humans, providing data to support research in a wide range of areas, including neuroscience, hepatology, obesity, and others.

Research Area: Neuro science; Hepatology; Obesity

Specification

Sample Type: Serum; Plasma; Urine; Tissue homogenates

Detection Range: 62.5-4000 pg/mL

Sensitivity: <15.6 pg/mL

Precision (Intra-assay): CV<10%

Precision (Inter-assay): CV<12%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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