Human peptide YY (PYY) ELISA kit-Quantitative competitive (31.25-2000 pg/mL)

Cat. No.: 0126WXX-1653

Assay Type: Quantitative competitive ELISA

Target Species: Human

Assay Target: PYY

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Human peptide YY (PYY) ELISA kit-Quantitative competitive (31.25-2000 pg/mL)

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Product Overview

Description: Human peptide YY (PYY) ELISA kit-Quantitative competitive (31.25-2000 pg/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of PYY in human samples with a range of 31.25-2000 pg/mL and a minimum detectable dose (sensitivity) of 18.75 pg/mL. The kit is highly sensitive and easy to use.

Assay Principle: This kit is based on the competitive ELISA detection method. The microtiter plate is pre-coated with the target antigen. During the reaction, the target analyte in the sample or standard competes with a fixed amount of immobilized target on the solid phase for binding sites on a biotinylated detection antibody specific to the target. After washing away excess conjugate and unbound samples, HRP-streptavidin is added. Following incubation, TMB substrate is introduced. The enzyme-substrate reaction is terminated with a sulphuric acid solution, and the absorbance is measured at 450 nm. The target concentration is determined by comparing the sample O.D. to the standard curve.

Background: PYY (Peptide YY), a critical gut peptide and member of the NPY family located at chromosomal position 17q21.1, serves as a fundamental regulator in the physiological landscape of obesity and digestive homeostasis. Secreted into the extracellular region as two distinct isoforms through alternative splicing, this neuropeptide hormone exerts its biological influence primarily through G-protein coupled receptor signaling pathways to modulate eating behavior and appetite regulation. Its multifaceted role in the gastrointestinal tract includes the inhibition of exocrine pancreatic secretion, the mediation of vasoconstrictory actions, and the suppression of jejunal and colonic motility, thereby slowing digestion to enhance satiety. Beyond its classical endocrine functions in feeding behavior, PYY is also implicated in broader cellular processes such as cell proliferation and cytoskeleton organization.

Synonyms: Peptide YY-like

Applications: Human peptide YY (PYY) ELISA kit-Quantitative competitive (31.25-2000 pg/mL) is used to quantify PYY in serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples of human, providing data to support research in a wide range of areas, including neuroscience, hepatology, obesity, and others.

Research Area: Neuro science; Hepatology; Obesity

Specification

Sample Type: Serum; Plasma; Cell culture supernatant; Cell or tissue lysate; Other liquid samples

Detection Range: 31.25-2000 pg/mL

Sensitivity: 18.75 pg/mL

Precision (Intra-assay): CV<8%

Precision (Inter-assay): CV<10%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at -20°C (microplate; standard) and 2-8°C (other reagents).

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