Human pancreatic lipase (PL) ELISA kit (0.312-20 ng/mL)

Cat. No.: 0126WXX-1326

Assay Type: Quantitative sandwich ELISA

Target Species: Human

Assay Target: PL

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Human pancreatic lipase (PL) ELISA kit (0.312-20 ng/mL)

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Product Overview

Description: Human pancreatic lipase (PL) ELISA kit (0.312-20 ng/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of PL in human samples with a range of 0.312-20 ng/mL and a minimum detectable dose (sensitivity) of 0.06 ng/mL. The kit is highly sensitive and easy to use.

Assay Principle: This kit utilizes the double-antibody sandwich ELISA technique. The microtiter plate is pre-coated with an anti-human PL monoclonal antibody, and a biotinylated polyclonal antibody serves as the detection agent. Samples and biotinylated antibodies are added to the wells and washed with PBS or TBS. Avidin-peroxidase conjugates are then added to the wells. After the enzyme conjugate is thoroughly washed away, TMB substrate is introduced for coloration. TMB reacts via peroxidase activity to form a blue product, which finally turns yellow after the addition of the stop solution (color reagent C). The color intensity and the quantity of the target analyte in the sample are positively correlated.

Background: PL, a key member of the lipase gene family located at chromosomal position 10q26.1, is a specialized carboxyl esterase (EC 3.1.1.3) specifically expressed in the pancreas and secreted into the extracellular region. This hydrolase is essential for the efficient digestion of dietary fats, specifically driving the hydrolysis of insoluble, emulsified triglycerides into absorbable components. Beyond its primary role in lipid catabolism and cholesterol absorption, PNLIP is involved in retinoid metabolic processes and is the central molecular driver in Pancreatic Lipase Deficiency, making it a fundamental target for understanding gastrointestinal lipid digestion and its systemic impact on energy balance.

Synonyms: PNLIP; PTL; Lipase; pancreatic; Pancreatic triacylglycerol lipase

Applications: Human pancreatic lipase (PL) ELISA kit (0.312-20 ng/mL) is used to quantify PL in human serum, plasma, cell culture supernatant, and other sources, providing data to support research in a wide range of areas, including enzyme and kinase, metabolic pathway, Infection immunity, hepatology, obesity, and others.

Research Area: Enzyme & Kinase; Metabolic pathway; Infection immunity; Hepatology; Obesity

Specification

Sample Type: Human serum; Plasma; Cell culture supernatant; Organizations

Detection Range: 0.312-20 ng/mL

Sensitivity: 0.06 ng/mL

Precision (Intra-assay): CV≤8%

Precision (Inter-assay): CV≤12%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Recovery: 70-110%

Storage: Store at 2-8°C.

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