Human pancreatic lipase (PL) ELISA kit (0.3-90 ng/mL)

Cat. No.: 0126WXX-1344

Assay Type: Quantitative sandwich ELISA

Target Species: Human

Assay Target: PL

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Human pancreatic lipase (PL) ELISA kit (0.3-90 ng/mL)

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Product Overview

Description: Human pancreatic lipase (PL) ELISA kit (0.3-90 ng/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of PL in human samples with a range of 0.3-90 ng/mL and a minimum detectable dose (sensitivity) of 0.152 ng/mL. The kit is highly sensitive and easy to use.

Assay Principle: The microplate is pre-coated with a human PL antibody. PL present in the sample binds to the immobilized capture antibodies. Subsequently, a biotinylated human PL detection antibody is added, followed by streptavidin-HRP. After incubation and a thorough wash step, a substrate solution is introduced. Color develops in direct proportion to the amount of human PL present. The reaction is terminated with an acidic stop solution, and the absorbance is measured at 450 nm.

Background: PL, a key member of the lipase gene family located at chromosomal position 10q26.1, is a specialized carboxyl esterase (EC 3.1.1.3) specifically expressed in the pancreas and secreted into the extracellular region. This hydrolase is essential for the efficient digestion of dietary fats, specifically driving the hydrolysis of insoluble, emulsified triglycerides into absorbable components. Beyond its primary role in lipid catabolism and cholesterol absorption, PNLIP is involved in retinoid metabolic processes and is the central molecular driver in Pancreatic Lipase Deficiency, making it a fundamental target for understanding gastrointestinal lipid digestion and its systemic impact on energy balance.

Synonyms: PNLIP; PTL; Lipase; pancreatic; Pancreatic triacylglycerol lipase

Applications: Human pancreatic lipase (PL) ELISA kit (0.3-90 ng/mL) is used to quantify PL in serum, plasma, cell culture supernatant, cell lysates, and tissue homogenates of human, providing data to support research in a wide range of areas, including enzyme and kinase, metabolic pathway, Infection immunity, hepatology, obesity, and others.

Research Area: Enzyme & Kinase; Metabolic pathway; Infection immunity; Hepatology; Obesity

Specification

Sample Type: Serum; Plasma; Cell culture supernatants; Cell lysates; Tissue homogenates

Detection Range: 0.3-90 ng/mL

Sensitivity: 0.152 ng/mL

Precision (Intra-assay): CV<8%

Precision (Inter-assay): CV<10%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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