Human adiponectin (ADP) ELISA kit (1.563-100 ng/mL)

Cat. No.: OB0525WXX-260

Assay Type: Quantitative sandwich ELISA

Target Species: Human

Assay Target: ADP

Size:

Human adiponectin (ADP) ELISA kit (1.563-100 ng/mL)

INQUIRY Datasheet MSDS

Add to basket

Product Overview

Description: Human adiponectin (ADP) ELISA kit (1.563-100 ng/mL) is a ready-to-use ELISA kit for analyzing human ADP levels with a detection range of 1.563-100 ng/mL and a sensitivity of 0.938 ng/mL.

Assay Principle: The kit is based on a sandwich ELISA technique. The capture antibody is pre-coated on a 96-well plate. A biotin-conjugated antibody is used as the detection antibody. Standards, test samples, and biotin-conjugated detection antibodies are then added to the wells and washed with buffer. HRP-streptavidin is added, and the unbound conjugate is washed away with washing solution. TMB substrate is used to visualize the HRP enzyme reaction. TMB produces a blue product catalyzed by HRP, which turns yellow upon addition of an acidic termination solution. The density of the yellow color is proportional to the amount of target sample captured in the plate. The concentration of the target is calculated by reading the absorbance at 450 nm with a microplate reader.

Background: ADP is an important adipokine involved in the regulation of fat metabolism and insulin sensitivity, exhibiting antidiabetic, anti-atherosclerotic, and anti-inflammatory properties. It increases glucose utilization and fatty acid burning, stimulates AMPK phosphorylation and activation in liver and skeletal muscle, and may play a role in cell growth, angiogenesis, and tissue remodeling.

Synonyms: ADIPOQ; ACDC; APM1; Adipocyte C1q and collagen domain-containing protein; ACRP30; Adipose most abundant gene transcript 1 protein; apM-1; Gelatin-binding protein; Adiponectin

Applications: Human adiponectin (ADP) ELISA kit (1.563-100 ng/mL) is designed for the in vitro quantitative analysis of ADP levels in human serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples.

Research Area: Hormone research; Lipid metabolism; Cell differentiation; Obesity

Specification

Sample Type: Serum; Plasma; Cell culture supernatant; Cell or tissue lysate; Other liquid samples

Detection Range: 1.563-100 ng/mL

Sensitivity: 0.938 ng/mL

Precision (Intra-assay): CV<8%

Precision (Inter-assay): CV<10%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at -20°C (microplate, standard) and 2-8°C (other reagents).

Related Products

Inquiry

Inquiry Basket

Delete selected Check Out