Human neuropeptide Y receptor Y5 (NPY5R) ELISA kit (0.312-20 ng/mL)

Cat. No.: 0126WXX-1142

Assay Type: Quantitative sandwich ELISA

Target Species: Human

Assay Target: NPY5R

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Human neuropeptide Y receptor Y5 (NPY5R) ELISA kit (0.312-20 ng/mL)

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Product Overview

Description: Human neuropeptide Y receptor Y5 (NPY5R) ELISA kit (0.312-20 ng/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of NPY5R in human samples with a range of 0.312-20 ng/mL and a minimum detectable dose (sensitivity) of 0.108 ng/mL. The kit is highly sensitive and easy to use.

Assay Principle: The microtiter plate included in this kit is pre-coated with an antibody specific to NPY5R. Standards or samples are introduced into the designated wells along with a biotin-conjugated antibody preparation specific for NPY5R. Next, avidin-conjugated HRP is added to each microplate well and incubated. Upon the addition of TMB substrate solution, only those wells containing the NPY5R-biotin-avidin sandwich complex will exhibit a colorimetric change. The enzymatic reaction is terminated with a sulphuric acid solution, and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 10 nm. The concentration of NPY5R in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Background: NPY5R is a G protein-coupled receptor that binds to neuropeptide Y (NPY) and peptide YY (PYY). These are key signaling molecules regulating appetite within the brain. When NPY5R is activated, it inhibits adenylate cyclase activity via its G protein, thereby triggering cellular responses. As a central control point regulating appetite and feeding behavior, it serves as a critical target in obesity research.

Synonyms: NPY-Y5 receptor

Applications: Human neuropeptide Y receptor Y5 (NPY5R) ELISA kit (0.312-20 ng/mL) is used to quantify NPY5R in tissue homogenates and other biological fluids of human, providing data to support research in a wide range of areas, including signal transduction, obesity, etc.

Research Area: Signal transduction; Obesity

Specification

Sample Type: Tissue homogenates; Other biological fluids

Detection Range: 0.312-20 ng/mL

Sensitivity: 0.108 ng/mL

Precision (Intra-assay): CV<10%

Precision (Inter-assay): CV<12%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 4°C (TMB substrate, wash buffer, stop solution) and -20°C (others).

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