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Human mu-crystallin homolog (CRYM) ELISA kit
Cat. No.:
OB0625WXX-468
Assay Type:
Quantitative sandwich ELISA
Target Species:
Human
Assay Target:
CRYM
Size:
Add to basket
Product Overview
Description:
Human mu-crystallin homolog (CRYM) ELISA kit is an ELISA-based in vitro research tool designed specifically for the quantitative detection of CRYM in human with a range of 0.25-8 ng/mL and a specificity of 0.1 ng/mL.
Assay Principle:
Solid phase antibody is made from purified human CRYM antibody, then CRYM and HRP-labeled CRYM antibody are added to the wells, and the reaction becomes an antibody-antigen-antibody-enzyme complex. After complete washing, the TMB substrate solution is added, and the TMB substrate turns blue, catalyzed by the HRP enzyme. Then sulfuric acid is added to terminate the reaction, and the change in color is measured spectrophotometrically at 450 nm. The color change is measured spectrophotometrically at 450 nm. The concentration of CRYM in the sample is then determined by comparing the OD of the sample with the standard curve.
Background:
CRYM specifically catalyzes the reduction of imine bonds in brain substrates. It may be involved in the regulation of the free intracellular concentration of triiodothyronine and access to its nuclear receptors.
Synonyms:
NADP-regulated thyroid-hormone-binding protein; THBP
Applications:
Human mu-crystallin homolog (CRYM) ELISA kit is used to quantify CRYM in serum, plasma, tissue homogenate, feces, urine samples of human, providing data to support a wide range of studies.
Research Area:
Endocrine
Specification
Sample Type:
Serum; Plasma; Tissue homogenate; Feces; Urine
Detection Range:
0.25-8 ng/mL
Sensitivity:
0.1 ng/mL
Precision (Intra-assay):
CV<15%
Precision (Inter-assay):
CV<15%
Cross-reactivity:
No significant cross-reactivity or interference was observed.
Storage:
Store at 2-8°C.