Human meprin A subunit alpha (MEP1A) ELISA kit (0.156-10 ng/mL)

Cat. No.: 0126WXX-1038

Assay Type: Quantitative sandwich ELISA

Target Species: Human

Assay Target: MEP1A

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Human meprin A subunit alpha (MEP1A) ELISA kit (0.156-10 ng/mL)

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Product Overview

Description: Human meprin A subunit alpha (MEP1A) ELISA kit (0.156-10 ng/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of MEP1A in human samples with a range of 0.156-10 ng/mL and a minimum detectable dose (sensitivity) of <0.063 ng/mL. The kit is highly sensitive and easy to use.

Assay Principle: This kit utilizes the sandwich enzyme immunoassay technique. The provided microtiter plate is pre-coated with an antibody specific to MEP1A. Standards or samples are introduced into the designated wells along with a biotin-conjugated detection antibody specific for MEP1a. Subsequently, avidin-conjugated HRP is added to each microplate well and incubated. Upon the addition of TMB substrate solution, color development occurs exclusively in wells containing the MEP1a-biotin-avidin sandwich complex. The enzymatic reaction is terminated with a sulphuric acid solution, and the color change is measured spectrophotometrically at 450 nm ± 10 nm. The concentration of MEP1a in the samples is determined by comparing the O.D. of the samples to the standard curve.

Background: MEP1A is a constituent subunit of the zinc-dependent metalloproteinase Meprin A, highly expressed primarily in the renal brush border and intestine. Its core function is as an endopeptidase that hydrolyzes peptide bonds, participating in protein digestion, processing specific peptides, and degrading the extracellular matrix (ECM). It is associated with glucose and insulin metabolism.

Synonyms: Endopeptidase-2; N-benzoyl-L-tyrosyl-P-amino-benzoic acid hydrolase subunit alpha; PABA peptide hydrolase; PPH alpha; PPHA

Applications: Human meprin A subunit alpha (MEP1A) ELISA kit (0.156-10 ng/mL) is used to quantify MEP1A in tissue homogenates, cell lysates, and other biological fluids of human, providing data to support research in a wide range of areas, including metabolism, etc.

Research Area: Metabolism