Human lipoprotein lipase (LPL) ELISA kit (0.781-50 ng/mL)

Cat. No.: 0126WXX-951

Assay Type: Quantitative sandwich ELISA

Target Species: Human

Assay Target: LPL

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Human lipoprotein lipase (LPL) ELISA kit (0.781-50 ng/mL)

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Product Overview

Description: Human lipoprotein lipase (LPL) ELISA kit (0.781-50 ng/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of LPL in human samples with a range of 0.781-50 ng/mL and a minimum detectable dose (sensitivity) of 0.469 ng/mL. The kit is highly sensitive and easy to use.

Assay Principle: This kit is based on sandwich ELISA technology. An anti-LPL antibody is pre-coated onto 96-well plates, and a biotin-conjugated anti-LPL antibody is used for detection. Standards, samples, and the detection antibody are added sequentially and washed. HRP-streptavidin is then introduced, and unbound conjugates are washed away. TMB substrate is used to visualize the HRP enzymatic reaction, producing a yellow color upon acidification. The density of the yellow color is proportional to the LPL concentration captured in the plate, measured at 450 nm.

Background: LPL is an enzyme belonging to the lipoprotein lipase family. Its primary function is to break down triglycerides (a type of fat) in the blood. Specifically, LPL hydrolyzes triglycerides present in circulating lipoproteins such as chylomicrons and very low-density lipoproteins (VLDL). The free fatty acids and monoglycerides released during this process can be utilized by cells to generate energy or stored for later use. LPL's direct relevance to obesity research lies in its pivotal role in fat storage and metabolism. Given its central function in lipid metabolism, LPL has emerged as a key biological factor in obesity studies.

Synonyms: Clearing factor; Clearing factor lipase; EC 3.1.1.34

EC NO.: 3.1.1.34

Applications: Human lipoprotein lipase (LPL) ELISA kit (0.781-50 ng/mL) is used to quantify LPL in serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples of human, providing data to support research in a wide range of areas, including signal transduction, metabolism, obesity, etc.

Research Area: Signal Transduction; Metabolism; Obesity

Specification

Sample Type: Serum; Plasma; Cell culture supernatant; Cell or tissue lysate; Other liquid samples

Detection Range: 0.781-50 ng/mL

Sensitivity: 0.469 ng/mL

Precision (Intra-assay): CV<8%

Precision (Inter-assay): CV<10%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at -20°C (microplate, standard) and 2-8°C (other reagents).

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