Human lipopolysaccharide binding protein (LBP) ELISA kit (0.78-50 ng/mL)

Cat. No.: 0126WXX-763

Assay Type: Quantitative sandwich ELISA

Target Species: Human

Assay Target: LBP

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Human lipopolysaccharide binding protein (LBP) ELISA kit (0.78-50 ng/mL)

INQUIRY Datasheet MSDS

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Product Overview

Description: Human lipopolysaccharide binding protein (LBP) ELISA kit (0.78-50 ng/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of LBP in human samples with a range of 0.78-50 ng/mL and a minimum detectable dose (sensitivity) of 0.195 ng/mL. The kit is highly sensitive and easy to use.

Assay Principle: The microplate provided in this kit is pre-functionalized with a capture antibody specific to LBP. Standards or samples are introduced into the designated wells along with a biotin-labeled polyclonal detection antibody. Subsequently, avidin-conjugated HRP is added to each well and incubated. Following the addition of TMB substrate, only wells containing the LBP-biotin-avidin complex will undergo a colorimetric change. The enzymatic reaction is halted by a sulphuric acid solution, and the absorbance is recorded spectrophotometrically at 450 nm ± 2 nm. The LBP concentration is then calculated by comparing the sample O.D. values to the standard curve.

Background: LBP is a secreted protein primarily present in the extracellular space. Its core function is to bind bacterial lipopolysaccharide (LPS), a glycolipid found on the outer membrane of all Gram-negative bacteria. LBP is significant in obesity research because it plays a pivotal role in the link between inflammation, gut microbiota, and metabolic health. By studying LBP's mechanisms of action and its level changes, scientists can better understand how obesity leads to systemic inflammation and insulin resistance, potentially developing therapies targeting LBP or its associated signaling pathways.

Synonyms: LPS-binding protein

Applications: Human lipopolysaccharide binding protein (LBP) ELISA kit (0.78-50 ng/mL) is used to quantify LBP in serum, plasma, whole blood, tissue, saliva, urine, feces, and other fluids of human, providing data to support research in a wide range of areas, including Infection immunity, obesity, etc.

Research Area: Infection immunity; Obesity

Specification

Sample Type: Serum; Plasma; Whole blood; Other fluid; Tissue; Saliva; Urine; Feces

Detection Range: 0.78-50 ng/mL

Sensitivity: 0.195 ng/mL

Precision (Intra-assay): CV≤4.8%

Precision (Inter-assay): CV≤7.2%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Recovery: 0.88

Storage: Store at -20°C.

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