Human leptin receptor (LEPR) ELISA kit (2-600 ng/mL)

Cat. No.: 0126WXX-846

Assay Type: Quantitative sandwich ELISA

Target Species: Human

Assay Target: LEPR

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Human leptin receptor (LEPR) ELISA kit (2-600 ng/mL)

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Product Overview

Description: Human leptin receptor (LEPR) ELISA kit (2-600 ng/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of LEPR in human samples with a range of 2-600 ng/mL and a minimum detectable dose (sensitivity) of 0.9 ng/mL. The kit is highly sensitive and easy to use.

Assay Principle: The microplate is pre-coated with a human LEPR antibody. During the assay, LEPR in the sample binds to the immobilized antibodies. A biotinylated human LEPR detection antibody is then added to bind the captured antigen, followed by the addition of streptavidin-HRP. After incubation and a washing step to remove unbound reagents, a substrate solution is added. Color develops in proportion to the human LEPR concentration. The reaction is terminated with an acidic stop solution, and absorbance is read at 450 nm.

Background: LEPR, as a peptide hormone receptor, is responsible for receiving leptin signals. When leptin binds to LEPR, it initiates a series of intracellular cascades, including the positive regulation of the JAK-STAT signaling pathway and the positive regulation of the PI3K signaling pathway. These pathways are crucial for translating leptin signals into biological responses, such as appetite suppression and metabolic regulation. The relationship between LEPR and obesity is paramount. When leptin functions as the “satiety hormone,” LEPR acts as the “satiety switch” in the brain and other tissues. Without functional receptors, leptin signals cannot be received regardless of how high leptin concentrations become.

Synonyms: CD295; LEPR; OBR; LR

Applications: Human leptin receptor (LEPR) ELISA kit (2-600 ng/mL) is used to quantify LEPR in serum, plasma, cell culture supernatant, cell lysates, and tissue homogenates of human, providing data to support research in a wide range of areas, including signal transduction, CD & adhesion molecule, metabolic pathway, endocrinology, obesity, etc.

Research Area: Signal transduction; CD & Adhesion molecule; Metabolic pathway; Endocrinology; Obesity

Specification

Sample Type: Serum; Plasma; Cell culture supernatants; Cell lysates; Tissue homogenates

Detection Range: 2-600 ng/mL

Sensitivity: 0.9 ng/mL

Precision (Intra-assay): CV<8%

Precision (Inter-assay): CV<10%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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