Human insulin (INS) ELISA kit (7.813-500 pg/mL)

Cat. No.: 0126WXX-580

Assay Type: Quantitative sandwich ELISA

Target Species: Human

Assay Target: INS

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Human insulin (INS) ELISA kit (7.813-500 pg/mL)

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Product Overview

Description: Human insulin (INS) ELISA kit (7.813-500 pg/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of INS in human samples with a range of 7.813-500 pg/mL and a minimum detectable dose (sensitivity) of 4.688 pg/mL. The kit is highly sensitive and easy to use.

Assay Principle: This kit is based on sandwich ELISA technology utilizing an anti-INHB capture antibody pre-coated onto a 96-well plate. Biotin-conjugated anti-INHB is used as the detection antibody. Standards, samples, and the detection antibody are added sequentially, followed by a wash step and the addition of HRP-streptavidin. TMB substrates are used to visualize the HRP enzymatic reaction, producing a blue product that turns yellow after the addition of an acidic stop solution. The yellow color density is proportional to the amount of INHB captured, as measured by absorbance at 450 nm.

Background: INS is a hormone composed of an A chain and a B chain linked by two disulfide bonds. Secreted by the pancreas, it functions as a key regulator of metabolism within the body. Its primary role is to lower blood glucose levels by promoting glucose uptake into cells. INS is fundamentally linked to obesity through its role in energy metabolism and its relationship with insulin resistance. By targeting the insulin receptor signaling pathway and the diverse metabolic processes it regulates, researchers can develop novel anti-obesity therapies.

Synonyms: Pancreatic insulin

Applications: Human insulin (INS) ELISA kit (7.813-500 pg/mL) is used to quantify INS in serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples of human, providing data to support research in a wide range of areas, including metabolic pathway, endocrinology, hormone metabolism, obesity, etc.

Research Area: Metabolic pathway; Endocrinology; Hormone metabolism; Obesity

Specification

Sample Type: Serum; Plasma; Cell culture supernatant; Cell or tissue lysate; Other liquid samples

Detection Range: 7.813-500 pg/mL

Sensitivity: 4.688 pg/mL

Precision (Intra-assay): CV<8%

Precision (Inter-assay): CV<10%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at -20°C (microplate, standard) and 2-8°C (other reagents).

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