Horse growth hormone (GH) ELISA kit (0.312-20 ng/mL)

Cat. No.: 0126WXX-111

Assay Type: Quantitative competitive ELISA

Target Species: Horse

Assay Target: GH

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Horse growth hormone (GH) ELISA kit (0.312-20 ng/mL)

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Product Overview

Description: Horse growth hormone (GH) ELISA kit (0.312-20 ng/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of GH in horse samples with a range of 0.312-20 ng/mL and a minimum detectable dose (sensitivity) of 0.085 ng/mL. The kit is highly sensitive and easy to use.

Assay Principle: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to GH is pre-coated onto the microplate. A competitive reaction occurs between biotin-labeled GH and unlabeled GH from the sample. After incubation and washing, avidin-HRP is added. The amount of bound HRP conjugate and the resulting color intensity are inversely proportional to the concentration of GH in the sample. The final color is measured following substrate addition and reaction termination.

Background: Growth hormone (GH) is a crucial endocrine hormone that plays a key role in growth regulation and metabolic control. It primarily promotes bodily growth by stimulating the liver and other tissues to secrete insulin-like growth factor-1 (IGF-1), while also enhancing amino acid uptake and protein synthesis. Deficiencies in GH can lead to growth hormone deficiency disorders, such as dwarfism. Research indicates that GH induces lipolysis through direct lipolytic effects in adipose tissue and influences obesity to a certain extent.

Synonyms: GH1; GH-N; GHN; hGH-N; Somatotropin; Hygetropin; Jintropin; Kigtropin; Pituitary growth hormone; Growth hormone, normal

Applications: Horse growth hormone (GH) ELISA kit (0.312-20 ng/mL) is used to quantify GH in serum, plasma, and other biological fluids of horse, providing data to support research in a wide range of areas, including developmental biology, obesity, endocrinology, neuro science, metabolism, and others.

Research Area: Developmental biology; Obesity; Endocrinology; Neuro science; Metabolism

Specification

Sample Type: Serum; Plasma; Other biological fluids

Detection Range: 0.312-20 ng/mL

Sensitivity: 0.085 ng/mL

Precision (Intra-assay): CV<10%

Precision (Inter-assay): CV<12%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 4°C (TMB substrate, wash buffer, stop solution) and -20°C (other reagents).

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