Human insulin (INS) ELISA kit (0.2-60 mIU/L)

Cat. No.: 0126WXX-583

Assay Type: Quantitative sandwich ELISA

Target Species: Human

Assay Target: INS

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Human insulin (INS) ELISA kit (0.2-60 mIU/L)

INQUIRY Datasheet MSDS

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Product Overview

Description: Human insulin (INS) ELISA kit (0.2-60 mIU/L) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of INS in human samples with a range of 0.2-60 mIU/L and a minimum detectable dose (sensitivity) of 0.11 mIU/L. The kit is highly sensitive and easy to use.

Assay Principle: This ELISA utilizes a microplate pre-coated with a human INS capture antibody. Target INS in the sample binds to the immobilized antibody, followed by the sequential addition of a biotinylated human INS detection antibody and a streptavidin-HRP conjugate. After incubation and a washing step to remove unbound streptavidin-HRP, a substrate solution is added to produce a color change in direct proportion to the amount of human INS. The reaction is halted with an acidic stop solution, and the absorbance is measured at 450 nm.

Background: INS is a hormone composed of an A chain and a B chain linked by two disulfide bonds. Secreted by the pancreas, it functions as a key regulator of metabolism within the body. Its primary role is to lower blood glucose levels by promoting glucose uptake into cells. INS is fundamentally linked to obesity through its role in energy metabolism and its relationship with insulin resistance. By targeting the insulin receptor signaling pathway and the diverse metabolic processes it regulates, researchers can develop novel anti-obesity therapies.

Synonyms: Pancreatic insulin

Applications: Human insulin (INS) ELISA kit (0.2-60 mIU/L) is used to quantify INS in serum, plasma, cell culture supernatant, cell lysates, and tissue homogenates of human, providing data to support research in a wide range of areas, including metabolic pathway, endocrinology, hormone metabolism, obesity, etc.

Research Area: Metabolic pathway; Endocrinology; Hormone metabolism; Obesity

Specification

Sample Type: Serum; Plasma; Cell culture supernatants; Cell lysates; Tissue homogenates

Detection Range: 0.2-60 mIU/L

Sensitivity: 0.11 mIU/L

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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